{"id":128100,"date":"2022-07-29T07:57:36","date_gmt":"2022-07-29T07:57:36","guid":{"rendered":"https:\/\/www.cytena.com\/?post_type=application-notes&#038;p=128100"},"modified":"2025-09-16T11:18:49","modified_gmt":"2025-09-16T11:18:49","slug":"b-sight-sequencing-single-cell-genomes-of-uncultivated-bacteria-in-the-human-oral-microbiome","status":"publish","type":"application-notes","link":"https:\/\/www.cytena.com\/resource-hub\/application-note\/b-sight-sequencing-single-cell-genomes-of-uncultivated-bacteria-in-the-human-oral-microbiome\/","title":{"rendered":"B.SIGHT\u2122 \u2013 Sequencing Single-cell Genomes of Uncultivated Bacteria in the Human Oral Microbiome"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"128100\" class=\"elementor elementor-128100\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"application-notes\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d88d667 e-flex e-con-boxed e-con e-parent\" data-id=\"d88d667\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-545ef91 elementor-widget elementor-widget-text-editor\" data-id=\"545ef91\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"176\" data-end=\"628\">Uncultivated bacteria in the human oral microbiome remain largely unexplored, offering opportunities for new discoveries. In this study, the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/b-sight\/\">B.SIGHT<\/a> enabled label-free single-cell isolation with image-based detection, followed by miniaturized MDA that reduced reaction volumes and costs. Subsequent 16S rRNA sequencing allowed taxonomic classification, and because all steps were performed in standard microwell plates, the workflow scaled efficiently to thousands of organisms without cultivation.<\/p><p data-start=\"176\" data-end=\"628\">The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/b-sight\/\">B.SIGHT<\/a> was essential for precise and cost-effective sequencing of single-cell genomes in the oral microbiome.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0015a95 e-flex e-con-boxed e-con e-parent\" data-id=\"0015a95\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dce37eb elementor-widget elementor-widget-text-editor\" data-id=\"dce37eb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Learn more about how:<\/strong><\/p>\n<ul>\n<li data-start=\"781\" data-end=\"895\">\n<p data-start=\"783\" data-end=\"895\">Label-free single-cell isolation with image confirmation gives access to uncultivated oral microbiome bacteria<\/p>\n<\/li>\n<li data-start=\"896\" data-end=\"958\">\n<p data-start=\"898\" data-end=\"958\">Miniaturized MDA reduces reaction volumes and lowers costs<\/p>\n<\/li>\n<li data-start=\"959\" data-end=\"1040\">\n<p data-start=\"961\" data-end=\"1040\">Scalable workflows classify thousands of individual cells without cultivation<\/p>\n<\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Uncultivated bacteria in the human oral microbiome remain largely unexplored, offering opportunities for new discoveries. In this study, the B.SIGHT enabled label-free single-cell isolation with image-based detection, followed by miniaturized MDA that reduced reaction volumes and costs. Subsequent 16S rRNA sequencing allowed taxonomic classification, and because all steps were performed in standard microwell plates, the [&hellip;]<\/p>\n","protected":false},"featured_media":129097,"parent":0,"menu_order":0,"template":"elementor_theme","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"tags":[],"application_notes":[960,962],"class_list":["post-128100","application-notes","type-application-notes","status-publish","has-post-thumbnail","hentry","application_notes-microbiome","application_notes-single-cell-omics"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"Uncultivated bacteria in the human oral microbiome remain largely unexplored, offering opportunities for new discoveries. 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