{"id":128144,"date":"2022-07-29T10:58:31","date_gmt":"2022-07-29T10:58:31","guid":{"rendered":"https:\/\/www.cytena.com\/?post_type=application-notes&#038;p=128144"},"modified":"2025-09-16T11:21:07","modified_gmt":"2025-09-16T11:21:07","slug":"fast-miniaturized-and-automated-library-prep-for-high-quality-single-cell-rna-seq-data","status":"publish","type":"application-notes","link":"https:\/\/www.cytena.com\/resource-hub\/application-note\/fast-miniaturized-and-automated-library-prep-for-high-quality-single-cell-rna-seq-data\/","title":{"rendered":"Fast, Miniaturized and Automated Library Prep for High-quality Single-cell RNA-seq Data"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"128144\" class=\"elementor elementor-128144\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"application-notes\">\n\t\t\t\t<div class=\"elementor-element elementor-element-009c4e7 e-flex e-con-boxed e-con e-parent\" data-id=\"009c4e7\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ed911ef elementor-widget elementor-widget-text-editor\" data-id=\"ed911ef\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"96\" data-end=\"356\">The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight\/\">F.SIGHT\u2122<\/a> was used to isolate single HEK293FT cells into 384-well PCR plates, followed by automated miniaturized library preparation with the <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT\u2122<\/a>. Reaction volumes were reduced to one-tenth of the standard, enabling cost-efficient full-length scRNA-seq.<\/p><p data-start=\"358\" data-end=\"518\">This workflow shows how the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight\/\">F.SIGHT<\/a> together with the <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a> enable precise single-cell isolation and high-quality sequencing in a scalable, automated format.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-60950e8 e-flex e-con-boxed e-con e-parent\" data-id=\"60950e8\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5e688e4 elementor-widget elementor-widget-text-editor\" data-id=\"5e688e4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Learn more about how:<\/strong><\/p><ul><li data-start=\"535\" data-end=\"603\"><p data-start=\"537\" data-end=\"603\"><span style=\"font-size: 16px;\">The <\/span><a style=\"font-size: 16px; background-color: #ffffff;\" href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight\/\">F.SIGHT<\/a><span style=\"font-size: 16px;\"> isolates single cells with high precision<\/span><\/p><\/li><li data-start=\"535\" data-end=\"603\"><p data-start=\"537\" data-end=\"603\">The <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a> miniaturizes library preparation for cost efficiency<\/p><\/li><li data-start=\"535\" data-end=\"603\"><p data-start=\"537\" data-end=\"603\"><span style=\"font-size: 16px;\">Both systems streamline full-length scRNA-seq in an automated workflow<\/span><\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>The F.SIGHT\u2122 was used to isolate single HEK293FT cells into 384-well PCR plates, followed by automated miniaturized library preparation with the I.DOT\u2122. Reaction volumes were reduced to one-tenth of the standard, enabling cost-efficient full-length scRNA-seq. This workflow shows how the F.SIGHT together with the I.DOT enable precise single-cell isolation and high-quality sequencing in a scalable, [&hellip;]<\/p>\n","protected":false},"featured_media":128145,"parent":0,"menu_order":0,"template":"elementor_theme","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"tags":[],"application_notes":[964,962],"class_list":["post-128144","application-notes","type-application-notes","status-publish","has-post-thumbnail","hentry","application_notes-genomics","application_notes-single-cell-omics"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"The F.SIGHT\u2122 was used to isolate single HEK293FT cells into 384-well PCR plates, followed by automated miniaturized library preparation with the I.DOT\u2122. Reaction volumes were reduced to one-tenth of the standard, enabling cost-efficient full-length scRNA-seq.This workflow shows how the F.SIGHT together with the I.DOT enable precise single-cell isolation and high-quality sequencing in a scalable, automated\" \/>\n\t\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t\t<link rel=\"canonical\" href=\"https:\/\/www.cytena.com\/resource-hub\/application-note\/fast-miniaturized-and-automated-library-prep-for-high-quality-single-cell-rna-seq-data\/\" \/>\n\t\t<meta name=\"generator\" content=\"All in One SEO Pro (AIOSEO) 4.3.0 \" \/>\n\t\t<meta property=\"og:locale\" content=\"en_US\" \/>\n\t\t<meta property=\"og:site_name\" content=\"CYTENA\" \/>\n\t\t<meta property=\"og:type\" content=\"article\" \/>\n\t\t<meta property=\"og:title\" content=\"Fast, Miniaturized and Automated Library Prep for High-quality Single-cell RNA-seq Data - CYTENA\" \/>\n\t\t<meta property=\"og:description\" content=\"The F.SIGHT\u2122 was used to isolate single HEK293FT cells into 384-well PCR plates, followed by automated miniaturized library preparation with the I.DOT\u2122. 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