{"id":128158,"date":"2022-07-29T11:20:33","date_gmt":"2022-07-29T11:20:33","guid":{"rendered":"https:\/\/www.cytena.com\/?post_type=application-notes&#038;p=128158"},"modified":"2025-09-16T11:20:50","modified_gmt":"2025-09-16T11:20:50","slug":"combined-single-cell-dispensing-and-3d-full-well-imaging-for-cell-lines-with-99-99-probability-of-monoclonality","status":"publish","type":"application-notes","link":"https:\/\/www.cytena.com\/resource-hub\/application-note\/combined-single-cell-dispensing-and-3d-full-well-imaging-for-cell-lines-with-99-99-probability-of-monoclonality\/","title":{"rendered":"Combined Single-cell Dispensing and 3D Full Well Imaging for Cell Lines with &gt;99.99% Probability of Monoclonality"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"128158\" class=\"elementor elementor-128158\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"application-notes\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1ae038d e-flex e-con-boxed e-con e-parent\" data-id=\"1ae038d\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a64a894 elementor-widget elementor-widget-text-editor\" data-id=\"a64a894\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"140\" data-end=\"623\">Ensuring monoclonality in cell line development is essential yet challenging. In this study, the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\">UP.SIGHT\u2122<\/a> combines double verification of single-cell dispensing, via nozzle imaging and novel 3D full-well imaging, to confirm monoclonality (&gt;99.99%) without centrifugation or fluorescent labeling. Compatible with 96-, 384-, and 1536-well plates, the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\">UP.SIGHT<\/a>\u00a0enables high-throughput single-cell cloning and colony tracking, with minimal hands-on time and increased efficiency.<\/p><p data-start=\"625\" data-end=\"756\">Our findings support the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\">UP.SIGHT<\/a>\u00a0as an all-in-one solution for rapid, accurate, and verifiable single-cell cloning workflows.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-5ed646a e-flex e-con-boxed e-con e-parent\" data-id=\"5ed646a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-23a9145 elementor-widget elementor-widget-text-editor\" data-id=\"23a9145\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Learn more about how:<br \/><\/strong><\/p><ul><li data-start=\"785\" data-end=\"884\"><p data-start=\"787\" data-end=\"884\">The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\">UP.SIGHT<\/a>\u00a0provides dual assurance of monoclonality with nozzle and 3D full-well imaging<\/p><\/li><li data-start=\"785\" data-end=\"884\"><p data-start=\"787\" data-end=\"884\">Imaging the full liquid volume eliminates the need for centrifugation and prevents ghost well artifacts<\/p><\/li><li data-start=\"993\" data-end=\"1090\"><p data-start=\"995\" data-end=\"1090\">Compatibility with multiple plate formats enables high-throughput cloning and colony tracking<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Ensuring monoclonality in cell line development is essential yet challenging. In this study, the UP.SIGHT\u2122 combines double verification of single-cell dispensing, via nozzle imaging and novel 3D full-well imaging, to confirm monoclonality (&gt;99.99%) without centrifugation or fluorescent labeling. Compatible with 96-, 384-, and 1536-well plates, the UP.SIGHT\u00a0enables high-throughput single-cell cloning and colony tracking, with minimal [&hellip;]<\/p>\n","protected":false},"featured_media":128159,"parent":0,"menu_order":0,"template":"elementor_theme","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"tags":[],"application_notes":[959,973,989,988,1022],"class_list":["post-128158","application-notes","type-application-notes","status-publish","has-post-thumbnail","hentry","application_notes-cell-line-development","application_notes-cell-therapy-stem-cells","application_notes-cell-based-assay","application_notes-gene-therapy","application_notes-monoclonal-antibodies"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"Ensuring monoclonality in cell line development is essential yet challenging. In this study, the UP.SIGHT\u2122 combines double verification of single-cell dispensing, via nozzle imaging and novel 3D full-well imaging, to confirm monoclonality (&gt;99.99%) without centrifugation or fluorescent labeling. 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