{"id":128177,"date":"2022-07-29T11:55:42","date_gmt":"2022-07-29T11:55:42","guid":{"rendered":"https:\/\/www.cytena.com\/?post_type=application-notes&#038;p=128177"},"modified":"2025-09-16T11:21:57","modified_gmt":"2025-09-16T11:21:57","slug":"tumor-spheroid-formation-using-the-i-dot","status":"publish","type":"application-notes","link":"https:\/\/www.cytena.com\/resource-hub\/application-note\/tumor-spheroid-formation-using-the-i-dot\/","title":{"rendered":"Tumor Spheroid Formation Using the I.DOT\u2122"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"128177\" class=\"elementor elementor-128177\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"application-notes\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5e91213 e-flex e-con-boxed e-con e-parent\" data-id=\"5e91213\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cc7caf3 elementor-widget elementor-widget-text-editor\" data-id=\"cc7caf3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"96\" data-end=\"356\">The <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a> was applied in the liquid overlay technique (LOT) to generate spheroids from the A549 lung cancer cell line. Cells aggregated into spheroids within days and were monitored for size and shape over 14 days.<\/p><p data-start=\"96\" data-end=\"356\">Compared to manual pipetting, the <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a> delivered precise cell numbers, producing spheroids with more uniform morphology up to ten times faster.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-3ad8f67 e-flex e-con-boxed e-con e-parent\" data-id=\"3ad8f67\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cfd12a8 elementor-widget elementor-widget-text-editor\" data-id=\"cfd12a8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Learn more about how:<\/strong><\/p><ul><li data-start=\"218\" data-end=\"293\"><p data-start=\"220\" data-end=\"293\">The <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a> supports precise dispensing with the liquid overlay technique<\/p><\/li><li data-start=\"294\" data-end=\"357\"><p data-start=\"296\" data-end=\"357\">Uniform and consistent spheroids are formed from A549 cells<\/p><\/li><li data-start=\"358\" data-end=\"419\"><p data-start=\"360\" data-end=\"419\">Dispensing is up to 10 times faster than manual pipetting<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>The I.DOT was applied in the liquid overlay technique (LOT) to generate spheroids from the A549 lung cancer cell line. Cells aggregated into spheroids within days and were monitored for size and shape over 14 days. Compared to manual pipetting, the I.DOT delivered precise cell numbers, producing spheroids with more uniform morphology up to ten [&hellip;]<\/p>\n","protected":false},"featured_media":128216,"parent":0,"menu_order":0,"template":"elementor_theme","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"tags":[],"application_notes":[966,989],"class_list":["post-128177","application-notes","type-application-notes","status-publish","has-post-thumbnail","hentry","application_notes-cancer-research","application_notes-cell-based-assay"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"The I.DOT was applied in the liquid overlay technique (LOT) to generate spheroids from the A549 lung cancer cell line. 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