{"id":139464,"date":"2023-02-07T08:21:58","date_gmt":"2023-02-07T08:21:58","guid":{"rendered":"https:\/\/www.cytena.com\/?post_type=application-notes&#038;p=139464"},"modified":"2025-09-16T12:12:42","modified_gmt":"2025-09-16T12:12:42","slug":"user-friendly-automation-of-full-length-single-cell-rna-sequencing-with-flash-seq","status":"publish","type":"application-notes","link":"https:\/\/www.cytena.com\/resource-hub\/application-note\/user-friendly-automation-of-full-length-single-cell-rna-sequencing-with-flash-seq\/","title":{"rendered":"User-friendly automation of full-length single-cell RNA-sequencing with FLASH-seq"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"139464\" class=\"elementor elementor-139464\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"application-notes\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2b6f1b5 e-flex e-con-boxed e-con e-parent\" data-id=\"2b6f1b5\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a825ca6 elementor-widget elementor-widget-text-editor\" data-id=\"a825ca6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"176\" data-end=\"628\">Week-22 human retinal organoids were processed with an automated FLASH-seq workflow. The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight-omics\/\">F.SIGHT\u2122 OMICS<\/a> enabled precise single-cell isolation, the <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT\u2122<\/a> ensured miniaturized reagent dispensing, and the <a href=\"https:\/\/www.cytena.com\/products\/microplate-washers-and-dispensers\/c-wash\/\">C.WASH\u2122<\/a> provided tip-free purification. Together, these systems made full-length scRNA-seq more efficient, reducing manual effort while ensuring high-quality data.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9907f1d e-flex e-con-boxed e-con e-parent\" data-id=\"9907f1d\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-40dc785 elementor-widget elementor-widget-text-editor\" data-id=\"40dc785\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Learn more about how:<\/strong><\/p><ul><li data-start=\"510\" data-end=\"570\"><p data-start=\"512\" data-end=\"570\"><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight-omics\/\">F.SIGHT OMICS<\/a> enabled precise single-cell isolation<\/p><\/li><li data-start=\"571\" data-end=\"629\"><p data-start=\"573\" data-end=\"629\"><a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a>\u00a0streamlined miniaturized reagent dispensing<\/p><\/li><li data-start=\"630\" data-end=\"692\"><p data-start=\"632\" data-end=\"692\"><a href=\"https:\/\/www.cytena.com\/products\/microplate-washers-and-dispensers\/c-wash\/\">C.WASH<\/a>\u00a0improved purification with tip-free automation<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Week-22 human retinal organoids were processed with an automated FLASH-seq workflow. The F.SIGHT\u2122 OMICS enabled precise single-cell isolation, the I.DOT\u2122 ensured miniaturized reagent dispensing, and the C.WASH\u2122 provided tip-free purification. Together, these systems made full-length scRNA-seq more efficient, reducing manual effort while ensuring high-quality data. Learn more about how: F.SIGHT OMICS enabled precise single-cell isolation [&hellip;]<\/p>\n","protected":false},"featured_media":139467,"parent":0,"menu_order":0,"template":"elementor_theme","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"unboxed","site-sidebar-style":"unboxed","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"tags":[],"application_notes":[976,962],"class_list":["post-139464","application-notes","type-application-notes","status-publish","has-post-thumbnail","hentry","application_notes-rna-sequencing","application_notes-single-cell-omics"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"Week-22 human retinal organoids were processed with an automated FLASH-seq workflow. The F.SIGHT\u2122 OMICS enabled precise single-cell isolation, the I.DOT\u2122 ensured miniaturized reagent dispensing, and the C.WASH\u2122 provided tip-free purification. Together, these systems made full-length scRNA-seq more efficient, reducing manual effort while ensuring high-quality data.Learn more about how:F.SIGHT OMICS enabled precise single-cell isolationI.DOT streamlined miniaturized reagent\" \/>\n\t\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t\t<link rel=\"canonical\" href=\"https:\/\/www.cytena.com\/resource-hub\/application-note\/user-friendly-automation-of-full-length-single-cell-rna-sequencing-with-flash-seq\/\" \/>\n\t\t<meta name=\"generator\" content=\"All in One SEO Pro (AIOSEO) 4.3.0 \" \/>\n\t\t<meta property=\"og:locale\" content=\"en_US\" \/>\n\t\t<meta property=\"og:site_name\" content=\"CYTENA\" \/>\n\t\t<meta property=\"og:type\" content=\"article\" \/>\n\t\t<meta property=\"og:title\" content=\"User-friendly automation of full-length single-cell RNA-sequencing with FLASH-seq - CYTENA\" \/>\n\t\t<meta property=\"og:description\" content=\"Week-22 human retinal organoids were processed with an automated FLASH-seq workflow. 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