{"id":146439,"date":"2024-07-18T08:42:00","date_gmt":"2024-07-18T08:42:00","guid":{"rendered":"https:\/\/www.cytena.com\/?p=146439"},"modified":"2024-10-07T10:37:43","modified_gmt":"2024-10-07T10:37:43","slug":"maximizing-efficiency-in-cell-line-development-techniques-and-tips","status":"publish","type":"post","link":"https:\/\/www.cytena.com\/resource-hub\/blog\/maximizing-efficiency-in-cell-line-development-techniques-and-tips\/","title":{"rendered":"Maximizing Efficiency in Cell Line Development: Techniques and Tips"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"146439\" class=\"elementor elementor-146439\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-32535686 elementor-section-full_width elementor-section-content-middle elementor-section-height-default elementor-section-height-default\" data-id=\"32535686\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;gradient&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-32cd17b8\" data-id=\"32cd17b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-181c155a elementor-widget__width-initial elementor-widget elementor-widget-post-info\" data-id=\"181c155a\" data-element_type=\"widget\" data-widget_type=\"post-info.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-inline-items elementor-icon-list-items elementor-post-info\">\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-4ac82da elementor-inline-item\" itemprop=\"datePublished\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-date-svg<\/title><style>\t\t.s0 { opacity: .9;fill: #f8fea0 } \t\t.s1 { opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0\" d=\"m0 5.5c0-1.5 1.2-2.8 2.7-2.8h18.6c1.5 0 2.7 1.3 2.7 2.8v2.7h-24z\"><\/path><path id=\"Layer\" class=\"s1\" d=\"m2.7 3.6h18.6c1.1 0 1.9 0.8 1.9 1.9v15.8c0 1.1-0.8 1.9-1.9 1.9h-18.6c-1 0-1.9-0.8-1.9-1.9v-15.8c0-1.1 0.9-1.9 1.9-1.9z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m4.9 0h2.7v2.7h-2.7z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m16.4 0h2.7v2.7h-2.7z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-date\">\n\t\t\t\t\t\t\t\t\t\t<time>July 18, 2024<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-dd7bde9 elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-time-svg<\/title><style>\t\t.s0_{ opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0_\" d=\"m12.5 23.2c-6.2 0-11.2-5-11.2-11.2 0-6.2 5-11.2 11.2-11.2 6.2 0 11.2 5 11.2 11.2 0 6.2-5 11.2-11.2 11.2z\"><\/path><path id=\"Layer\" fill-rule=\"evenodd\" class=\"s0_\" d=\"m12.2 7.6v4.4l4.4 4.4\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-time\">\n\t\t\t\t\t\t\t\t\t\t<time>8:42 am<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38c517fb elementor-widget elementor-widget-heading\" data-id=\"38c517fb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Maximizing Efficiency in Cell Line Development: Techniques and Tips<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div data-ep-wrapper-link=\"{&quot;url&quot;:&quot;https:\\\/\\\/www.cytena.com\\\/resource-hub\\\/blog\\\/authors\\\/&quot;,&quot;is_external&quot;:&quot;&quot;,&quot;nofollow&quot;:&quot;&quot;,&quot;custom_attributes&quot;:&quot;&quot;}\" style=\"cursor: pointer\" class=\"bdt-element-link elementor-element elementor-element-714cfb2d elementor-widget elementor-widget-author-box\" data-id=\"714cfb2d\" data-element_type=\"widget\" data-widget_type=\"author-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-author-box\">\n\t\t\t\t\t\t\t<div  class=\"elementor-author-box__avatar\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/Untitled-design-2024-08-30T155852223-100x100.png\" alt=\"Picture of By             Fernando Aprile Garc\u00eda\" loading=\"lazy\">\n\t\t\t\t<\/div>\n\t\t\t\n\t\t\t<div class=\"elementor-author-box__text\">\n\t\t\t\t\t\t\t\t\t<div >\n\t\t\t\t\t\t<h4 class=\"elementor-author-box__name\">\n\t\t\t\t\t\t\tBy             Fernando Aprile Garc\u00eda\t\t\t\t\t\t<\/h4>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-author-box__bio\">\n\t\t\t\t\t\t<p>Head of 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class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-80b7311\" data-id=\"80b7311\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-767c0caf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"767c0caf\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5c7763e0\" data-id=\"5c7763e0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d5bc093 elementor-widget elementor-widget-heading\" data-id=\"3d5bc093\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Maximizing Efficiency in Cell Line Development: Techniques and Tips<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-54521cfb elementor-widget elementor-widget-text-editor\" data-id=\"54521cfb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell line development is essential for producing many modern therapies, including complex molecules and cell-based therapies<sup>1<\/sup>. New automation and <a href=\"https:\/\/www.cytena.com\/resource-hub\/blog\/gene-editing-in-cell-line-development-cutting-edge-approaches\/\">genetic engineering technologies<\/a> have made cell line development increasingly competitive. This acceleration in capabilities allows companies to bring novel therapeutics to market more quickly, giving companies that embrace the latest advances an edge over the competition<sup>2<\/sup>. Furthermore, faster, more efficient cell development can lead to incredible savings as companies traverse the expensive drug discovery pipeline within shorter timeframes<sup>3<\/sup>. Thus, companies must prioritize efficiency to achieve their goals. In recent years, we have witnessed improvements across all techniques that make up cell line development pipelines, including cell culture and screening. This article will explore how companies can maximize efficiency and continuously shorten their cell line development timelines.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-67a36380 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"67a36380\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-56382804\" data-id=\"56382804\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7f84821e elementor-widget elementor-widget-heading\" data-id=\"7f84821e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Automation<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b3f27eb elementor-widget elementor-widget-text-editor\" data-id=\"1b3f27eb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tAutomation is revolutionizing the biomedical industry by streamlining processes across basic research and industrial applications<sup4<\/sup>. Automation uses robotics, software, artificial intelligence, and modern fluid-handling technologies to make cell line development faster and less risky. Automation is a fundamental aspect of modern biomedicine, enhancing the efficiency of all techniques that incorporate it. Automated instruments like the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT from CYTENA<\/a> relieves scientists of repetitive, time-consuming tasks, allowing them to allocate more time to improving processes and analyzing data.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-77a1a3f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"77a1a3f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-4f0ee7f4\" data-id=\"4f0ee7f4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-495b568b elementor-widget elementor-widget-heading\" data-id=\"495b568b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Reduced Risk<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1eab2091 elementor-widget elementor-widget-text-editor\" data-id=\"1eab2091\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Cell line development processes require single-cell dispensing, which is time-consuming and prone to error and contamination <a href=\"https:\/\/www.cytena.com\/resource-hub\/blog\/gene-editing-in-cell-line-development-cutting-edge-approaches\/\">when performed manually<\/a><sup>5<\/sup>. Loss of clones can be devastating for cell line development, often requiring scientists to start from scratch. Clonal lines can fail due to instability and contamination, which can come from microorganisms or other clones. Automated dispensing reduces contamination by eliminating the need for manual input. Dispensing using the UP.SIGHT is more gentle on cells than other dispensing methods, which can<a href=\"https:\/\/www.cytena.com\/resource-hub\/customers\/cellistic\/\"> improve the stability and viability<\/a> of susceptible cell lines like induced pluripotent stem cells.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5072a011 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5072a011\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-48e82b38\" data-id=\"48e82b38\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6d1846b1 elementor-widget elementor-widget-heading\" data-id=\"6d1846b1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Higher Efficiency<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-46f09c0c elementor-widget elementor-widget-text-editor\" data-id=\"46f09c0c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tAutomation facilitates high throughput in cell line development by streamlining dispensing and screening processes. Manual pipetting, screening, and data management are incredibly time-consuming. Automation allows scientists to seed more clones and more efficiently select the highest-performing ones to proceed with. The UP.SIGHT\u2019s imaging technology, paired with the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/c-studio\/\">C.STUDIO software<\/a>, makes it easy for scientists to monitor clones over time, allowing them to pinpoint the best ones early and save resources when scaling up production (Fig. 1).\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2a40e97 elementor-widget elementor-widget-image\" data-id=\"2a40e97\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"750\" height=\"443\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-08.png\" class=\"attachment-large size-large wp-image-146455\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-08.png 750w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-08-300x177.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-08-600x354.png 600w\" sizes=\"(max-width: 750px) 100vw, 750px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-86f49a3 elementor-widget elementor-widget-text-editor\" data-id=\"86f49a3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<strong>Figure 1.<\/strong> The C.STUDIO software allows for accurate quantification of cell proliferation following single-cell seeding.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7fef8c64 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7fef8c64\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-561704bf\" data-id=\"561704bf\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6727e051 elementor-widget elementor-widget-heading\" data-id=\"6727e051\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Scaling<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ade68a3 elementor-widget elementor-widget-text-editor\" data-id=\"ade68a3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tChoosing when and how to scale up clones makes a huge difference in the overall efficiency of cell line development processes6. Ideally, researchers should use the least amount of resources possible without sacrificing efficiency and allocate resources toward selecting and propagating optimal clones. In practical terms, this means using a multiwell plate format to seed and grow clones and selecting high-performing clones as quickly as possible.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3f96fec elementor-widget elementor-widget-text-editor\" data-id=\"3f96fec\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMicrobioreactors offer small-scale culture and monitoring of cell lines during the early stages of development which are ideal for workflow efficiency. The <a href=\"https:\/\/www.cytena.com\/products\/microbioreactors\/s-nest\/\">S.NEST<\/a> from CYTENA is a microbioreactor that monitors pH and dissolved oxygen (DO) in real time allowing for precise control and user-defined optimization of culture conditions.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b0f88e5 elementor-widget elementor-widget-text-editor\" data-id=\"b0f88e5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tScaling up too early with poor-performing clones leads to a significant loss of resources and time, which increases exponentially the more clones are brought forward. The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT from CYTENA<\/a> allows scientists to seed single cells in 96- or 384-well formats in 2 and 8 minutes, respectively, facilitating high throughput cell line development. The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/c-studio\/\">C.STUDIO<\/a> allows the best clones to be selected early, meaning scientists optimize their scaling for speed and resource efficiency.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2a462b2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2a462b2\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-dc4e61f\" data-id=\"dc4e61f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2880e5b elementor-widget elementor-widget-heading\" data-id=\"2880e5b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Culture Conditions<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fa79901 elementor-widget elementor-widget-text-editor\" data-id=\"fa79901\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tOptimal cell culture conditions are essential for streamlining cell line development processes. Optimized conditions mean cells achieve high levels of proliferation and target protein production. If cells become overconfluent or are handled incorrectly, they can become stressed, which reduces proliferation and may impact productivity7. Furthermore, inconsistent culture conditions may lead to clonal instability and the accumulation of genetic changes that reduce productivity<sup>8<\/sup>.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dd406c1 elementor-widget elementor-widget-text-editor\" data-id=\"dd406c1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tInduced pluripotent stem cells (iPSCs), for example, are incredibly valuable in several research and therapeutic applications. However, they are very sensitive to culture conditions, including excessive handling. Research from CYTENA has found that adding supplements to growth media eliminated the need for media changes, which increased the clonal recovery rate and growth rate of iPSCs after seeding. <a href=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2023\/04\/UP.SIGHT-iPSC-Application-Note.pdf\">Read our application note to learn more<\/a>.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-62ec568 elementor-widget elementor-widget-text-editor\" data-id=\"62ec568\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tAutomated cell culture platforms remove much of the variation in cell culture that leads to these issues. The <a href=\"https:\/\/www.cytena.com\/products\/automated-cell-line-development\/c-station\/\">C.STATION from CYTENA<\/a> offers an all-in-one solution for cell line development. It incorporates the UP.SIGHT for single-cell dispensing and imaging with automated liquid handling and cell culture to ensure clones are grown in consistent user-defined conditions. The C.STATION monitors colony growth and performs media swaps as necessary to ensure optimal growth with full automation.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-3a25a46 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3a25a46\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-53448fe\" data-id=\"53448fe\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-080c5ac elementor-widget elementor-widget-heading\" data-id=\"080c5ac\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Screening<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b6aeb5d elementor-widget elementor-widget-text-editor\" data-id=\"b6aeb5d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tScreening is essential to the cell line development process. It ensures that cells are fit for purpose and discards poorly performing clones. The aim is to select high-performing cells that produce large quantities of the target protein. The best way to monitor this is to directly measure the amount of the protein that the cells produce.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-34faf71 elementor-widget elementor-widget-text-editor\" data-id=\"34faf71\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tThe <a href=\"https:\/\/www.cytena.com\/products\/reagents-consumables\/f-quant\/\">F.QUANT<\/a> is a dedicated assay from CYTENA that accurately measures the titer of target proteins produced by a cell. With the F.QUANT, scientists can ensure they select only the best cells to scale up and use for therapy production.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2ec2b64 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2ec2b64\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-54d9f6d\" data-id=\"54d9f6d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b4cba39 elementor-widget elementor-widget-heading\" data-id=\"b4cba39\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Quality Control<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-14a0ede elementor-widget elementor-widget-text-editor\" data-id=\"14a0ede\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tThere are lots of <a href=\"https:\/\/www.cytena.com\/resource-hub\/blog\/mammalian-cell-line-development-applications-and-common-challenges\/\">potential pitfalls<\/a> at every stage of cell line development. Therefore, keeping tabs on potential issues is essential for maximizing efficiency throughout the workflow. Robust quality control (QC) systems help ensure the reliability and consistency of cell lines, prevent contamination, verify genetic identity and stability, and ultimately maintain the productive capacity of cells.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-8890859 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8890859\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-99995b9\" data-id=\"99995b9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-12d6c20 elementor-widget elementor-widget-heading\" data-id=\"12d6c20\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Monoclonality<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5c1607a elementor-widget elementor-widget-text-editor\" data-id=\"5c1607a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tVerifying single-cell seeding is an early and essential QC step in cell line development. Demonstrable monoclonality is required for regulatory compliance and, therefore, for achieving success in therapy development. However, monoclonality can be challenging to prove, particularly with manual cell dispensing methods.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-57ff240 elementor-widget elementor-widget-text-editor\" data-id=\"57ff240\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tThe <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT from CYTENA<\/a> images cells as they are being dispensed and after they have settled in the well, giving scientists robust evidence of monoclonality and allowing them to progress to the next stages of cell line development with confidence (Fig. 2). The UP.SIGHT ensures a &gt;99.99% probability of clonal derivation and up to 80% clonal recovery rate, making it ideal for ambitious companies looking for a simple way to streamline their workflows.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9bbc9c8 elementor-widget elementor-widget-image\" data-id=\"9bbc9c8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"614\" height=\"706\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/immage-09.png\" class=\"attachment-large size-large wp-image-146456\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/immage-09.png 614w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/immage-09-261x300.png 261w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/immage-09-600x690.png 600w\" sizes=\"(max-width: 614px) 100vw, 614px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1284413 elementor-widget elementor-widget-text-editor\" data-id=\"1284413\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<strong>Figure 2.<\/strong> The UP.SIGHT uses microfluidics to gently dispense single cells and provides images to help demonstrate monoclonality to regulator bodies.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-d149968 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d149968\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-378dbec\" data-id=\"378dbec\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-22437b8 elementor-widget elementor-widget-heading\" data-id=\"22437b8\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Growth Issues<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ad25cd7 elementor-widget elementor-widget-text-editor\" data-id=\"ad25cd7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell viability and proliferation rate are essential factors to monitor throughout the cell line development process. However, researchers face many challenges when optimizing these parameters. Each cell line has specific requirements for optimal growth, so researchers must tailor their workflows accordingly. For instance, iPSCs are sensitive to multiple media swaps and seeding methods like FACS.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cbfcb28 elementor-widget elementor-widget-text-editor\" data-id=\"cbfcb28\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tRegardless of type, all cells are susceptible to contamination and require a continuous supply of nutrients for optimal growth. Manual handling methods increase the chances of contamination and can be incredibly time-consuming, especially when multiple clones are being cultured simultaneously. The UP.SIGHT and C.STUDIO allow scientists to track cell growth over time, making it easy to spot contamination and other issues that disrupt cell proliferation and viability.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-96c7cd1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"96c7cd1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-294c4968\" data-id=\"294c4968\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5a8b9f2d elementor-widget elementor-widget-heading\" data-id=\"5a8b9f2d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Conclusions and Future Trends<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-63c3a7d1 elementor-widget elementor-widget-text-editor\" data-id=\"63c3a7d1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMaximizing efficiency in cell line development is crucial for gaining and maintaining an advantage over the competition. Automation, monoclonality assurance, risk reduction, and optimized scaling significantly enhance cell line development processes. Instruments like the UP.SIGHT and integrated platforms like the C.STATION ensure consistency and high performance in cell culture, while tools like F.QUANT facilitate optimized screening processes. Future trends in cell line development will likely focus on further integration of artificial intelligence and machine learning to optimize processes, predictive analytics for better clone selection, and continuous advancements in automation. These innovations will drive faster, more cost-effective development, ultimately accelerating the delivery of novel therapeutics to market. Companies that remain aligned with the latest developments can expect rapid and sustained improvement in their cell line development processes.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1ac537dd elementor-widget elementor-widget-text-editor\" data-id=\"1ac537dd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Ready to future-proof your workflows while shrinking your cell line development timeline? CYTENA is ready to provide ambitious researchers with the tools they need to pioneer the next generation of therapies. <a href=\"https:\/\/www.cytena.com\/contact-and-support\/\">Contact one of our experts<\/a> to learn more about the UP.SIGHT, or <a href=\"https:\/\/www.cytena.com\/book-a-demo\/\">book a demo<\/a> today.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-98f2311 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"98f2311\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-39e671e\" data-id=\"39e671e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-66f6838 elementor-widget elementor-widget-heading\" data-id=\"66f6838\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">References<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7142fa5 elementor-widget elementor-widget-text-editor\" data-id=\"7142fa5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol><li>Tihanyi B, Nyitray L. Recent advances in CHO cell line development for recombinant protein production. Drug Discovery Today: Technologies. 2020;38:25-34. doi:10.1016\/j.ddtec.2021.02.003<\/li>\n<li>Clarke H, Mayer\u2010Bartschmid A, Zheng C, et al. When will we have a clone? An industry perspective on the typical CLD timeline. Biotechnology Progress. Published online March 13, 2024:e3449. doi:10.1002\/btpr.3449<\/li>\n<li>Schlander M, Hernandez-Villafuerte K, Cheng CY, Mestre-Ferrandiz J, Baumann M. How Much Does It Cost to Research and Develop a New Drug? A Systematic Review and Assessment. PharmacoEconomics. 2021;39(11):1243-1269. doi:10.1007\/s40273-021-01065-y<\/li>\n<li>Holland I, Davies JA. Automation in the Life Science Research Laboratory. Front Bioeng Biotechnol. 2020;8(571777). doi:10.3389\/fbioe.2020.571777<\/li>\n<li>Guan XL, Chang DPS, Mok ZX, Lee B. Assessing variations in manual pipetting: An under-investigated requirement of good laboratory practice. J Mass Spectrom Adv Clin Lab. 2023;30:25-29.<\/li> doi:10.1016\/j.jmsacl.2023.09.001\n<li>Karimi Alavijeh M, Lee YY, Gras SL. A perspective-driven and technical evaluation of machine learning in bioreactor scale-up: A case-study for potential model developments. Eng Life Sci. 2024;24(7):e2400023. doi:10.1002\/elsc.202400023<\/li>\n<li>Samoudi M, Masson HO, Kuo CC, Robinson CM, Lewis NE. From omics to Cellular mechanisms in mammalian cell factory development. Curr Opin Chem Eng. 2021;32:100688.<\/li> doi:10.1016\/j.coche.2021.100688\n<li>Wurm F, Wurm M. Cloning of CHO Cells, Productivity and Genetic Stability\u2014A Discussion. Processes. 2017;5(2):20. doi:10.3390\/pr5020020<\/li>\n<\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Linkedin Facebook X-twitter Instagram Maximizing Efficiency in Cell Line Development: Techniques and Tips Cell line development is essential for producing many modern therapies, including complex molecules and cell-based therapies1. New automation and genetic engineering technologies have made cell line development increasingly competitive. This acceleration in capabilities allows companies to bring novel therapeutics to market more [&hellip;]<\/p>\n","protected":false},"author":34,"featured_media":146440,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[992,991],"tags":[],"products_category":[],"class_list":["post-146439","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-resource-hub"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - 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