{"id":146442,"date":"2024-07-30T08:46:00","date_gmt":"2024-07-30T08:46:00","guid":{"rendered":"https:\/\/www.cytena.com\/?p=146442"},"modified":"2024-10-07T10:36:33","modified_gmt":"2024-10-07T10:36:33","slug":"cell-line-development-comprehensive-insights-and-advances","status":"publish","type":"post","link":"https:\/\/www.cytena.com\/resource-hub\/blog\/cell-line-development-comprehensive-insights-and-advances\/","title":{"rendered":"Cell Line Development: Comprehensive Insights and Advances"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"146442\" class=\"elementor elementor-146442\" 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data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-32535686 elementor-section-full_width elementor-section-content-middle elementor-section-height-default elementor-section-height-default\" data-id=\"32535686\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;gradient&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-32cd17b8\" data-id=\"32cd17b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-181c155a elementor-widget__width-initial elementor-widget elementor-widget-post-info\" data-id=\"181c155a\" data-element_type=\"widget\" data-widget_type=\"post-info.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-inline-items elementor-icon-list-items elementor-post-info\">\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-4ac82da elementor-inline-item\" itemprop=\"datePublished\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-date-svg<\/title><style>\t\t.s0 { opacity: .9;fill: #f8fea0 } \t\t.s1 { opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0\" d=\"m0 5.5c0-1.5 1.2-2.8 2.7-2.8h18.6c1.5 0 2.7 1.3 2.7 2.8v2.7h-24z\"><\/path><path id=\"Layer\" class=\"s1\" d=\"m2.7 3.6h18.6c1.1 0 1.9 0.8 1.9 1.9v15.8c0 1.1-0.8 1.9-1.9 1.9h-18.6c-1 0-1.9-0.8-1.9-1.9v-15.8c0-1.1 0.9-1.9 1.9-1.9z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m4.9 0h2.7v2.7h-2.7z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m16.4 0h2.7v2.7h-2.7z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-date\">\n\t\t\t\t\t\t\t\t\t\t<time>July 30, 2024<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-dd7bde9 elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-time-svg<\/title><style>\t\t.s0_{ opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0_\" d=\"m12.5 23.2c-6.2 0-11.2-5-11.2-11.2 0-6.2 5-11.2 11.2-11.2 6.2 0 11.2 5 11.2 11.2 0 6.2-5 11.2-11.2 11.2z\"><\/path><path id=\"Layer\" fill-rule=\"evenodd\" class=\"s0_\" d=\"m12.2 7.6v4.4l4.4 4.4\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-time\">\n\t\t\t\t\t\t\t\t\t\t<time>8:46 am<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38c517fb elementor-widget elementor-widget-heading\" data-id=\"38c517fb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Cell Line Development: Comprehensive Insights and Advances<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div data-ep-wrapper-link=\"{&quot;url&quot;:&quot;https:\\\/\\\/www.cytena.com\\\/resource-hub\\\/blog\\\/authors\\\/&quot;,&quot;is_external&quot;:&quot;&quot;,&quot;nofollow&quot;:&quot;&quot;,&quot;custom_attributes&quot;:&quot;&quot;}\" style=\"cursor: pointer\" class=\"bdt-element-link elementor-element elementor-element-714cfb2d elementor-widget elementor-widget-author-box\" data-id=\"714cfb2d\" data-element_type=\"widget\" data-widget_type=\"author-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-author-box\">\n\t\t\t\t\t\t\t<div  class=\"elementor-author-box__avatar\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/Untitled-design-2024-08-30T155852223-100x100.png\" alt=\"Picture of By             Fernando Aprile Garc\u00eda\" loading=\"lazy\">\n\t\t\t\t<\/div>\n\t\t\t\n\t\t\t<div class=\"elementor-author-box__text\">\n\t\t\t\t\t\t\t\t\t<div >\n\t\t\t\t\t\t<h4 class=\"elementor-author-box__name\">\n\t\t\t\t\t\t\tBy             Fernando Aprile Garc\u00eda\t\t\t\t\t\t<\/h4>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-author-box__bio\">\n\t\t\t\t\t\t<p>Head of Applications<\/p>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-39d9a17c\" data-id=\"39d9a17c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4638d6d0 elementor-widget elementor-widget-image\" data-id=\"4638d6d0\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"800\" height=\"534\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/6-1.png\" class=\"attachment-large size-large wp-image-146443\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/6-1.png 800w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/6-1-300x200.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/6-1-768x513.png 768w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/08\/6-1-600x401.png 600w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-365c5639 elementor-grid-4 e-grid-align-left social-icon-sticky elementor-widget__width-initial elementor-absolute e-grid-align-mobile-center elementor-shape-rounded elementor-widget elementor-widget-social-icons\" data-id=\"365c5639\" data-element_type=\"widget\" data-settings=\"{&quot;_position&quot;:&quot;absolute&quot;}\" data-widget_type=\"social-icons.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-social-icons-wrapper elementor-grid\">\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-linkedin elementor-repeater-item-8003c4c\" href=\"https:\/\/www.linkedin.com\/company\/cytena\/\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Linkedin<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-linkedin\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-facebook elementor-repeater-item-a7a9aa2\" href=\"https:\/\/www.facebook.com\/CYTENA1\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Facebook<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-facebook\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-x-twitter elementor-repeater-item-432fafb\" href=\"https:\/\/x.com\/cytena\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">X-twitter<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-x-twitter\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-instagram elementor-repeater-item-f07f607\" href=\"https:\/\/www.instagram.com\/cytena\/\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Instagram<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-instagram\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-620483af elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"620483af\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-80b7311\" data-id=\"80b7311\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-767c0caf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"767c0caf\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5c7763e0\" data-id=\"5c7763e0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3d5bc093 elementor-widget elementor-widget-heading\" data-id=\"3d5bc093\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Cell Line Development: Comprehensive Insights and Advances<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-54521cfb elementor-widget elementor-widget-text-editor\" data-id=\"54521cfb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell line development is a cornerstone of several modern biomedical applications. It provides essential models for studying diseases and tools for developing the latest therapeutic products<sup>1<\/sup>. Researchers leverage the unique benefits of mammalian cell lines and innovations in gene editing, such as clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 and prime editing, to continuously enhance cell line development processes<sup>2<\/sup>. Advancements in automation technology are maximizing efficiency in cell line development by enabling higher throughput, reducing contamination risks, and simplifying the selection of high-performing clones<sup>3<\/sup>. These developments are transforming cell line development, making it more reliable and effective than ever. This article covers applications, innovations, and challenges in cell line development and introduces <a href=\"https:\/\/www.cytena.com\/products\/\">CYTENA\u2019s instruments<\/a> as future-proof solutions for therapy development.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-67a36380 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"67a36380\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-56382804\" data-id=\"56382804\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7f84821e elementor-widget elementor-widget-heading\" data-id=\"7f84821e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Mammalian Cell Line Development<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b3f27eb elementor-widget elementor-widget-text-editor\" data-id=\"1b3f27eb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMammalian cell line development is essential for both translational and basic biomedical research. It offers cost-effective and manageable models for various diseases and tools for the production of cutting-edge therapeutics<sup>1,4<\/sup>.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-77a1a3f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"77a1a3f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-4f0ee7f4\" data-id=\"4f0ee7f4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-495b568b elementor-widget elementor-widget-heading\" data-id=\"495b568b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Applications<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1eab2091 elementor-widget elementor-widget-text-editor\" data-id=\"1eab2091\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tVirtually all fields in biomedicine benefit from mammalian cell line development, including recombinant protein production, vaccine production, cell and gene therapies, drug discovery and development, and fundamental research.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5072a011 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5072a011\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-48e82b38\" data-id=\"48e82b38\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6d1846b1 elementor-widget elementor-widget-heading\" data-id=\"6d1846b1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Drug Discovery<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-46f09c0c elementor-widget elementor-widget-text-editor\" data-id=\"46f09c0c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMammalian cell lines provide a reproducible system for monitoring cellular responses to drugs and are crucial for high-throughput screening of drug candidates, biomarker discovery, and personalized medicine applications<sup>5,6<\/sup>.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-15629e8f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"15629e8f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-2630525d\" data-id=\"2630525d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-75bdb138 elementor-widget elementor-widget-heading\" data-id=\"75bdb138\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Therapy Production<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3c47d64c elementor-widget elementor-widget-text-editor\" data-id=\"3c47d64c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMammalian cell line development enables the production of complex molecules and antibodies, which are essential biological therapies for a growing list of indications. Technologies like the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT single-cell dispenser and imager<\/a> ensure high clonal derivation in cell line development, significantly de-risking therapy production pipelines (Fig. 1).\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-234f25e elementor-widget elementor-widget-image\" data-id=\"234f25e\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"536\" height=\"380\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-10.png\" class=\"attachment-large size-large wp-image-146460\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-10.png 536w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-10-300x213.png 300w\" sizes=\"(max-width: 536px) 100vw, 536px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-31d6bd6 elementor-widget elementor-widget-text-editor\" data-id=\"31d6bd6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<strong>Figure 1.<\/strong> The UP.SIGHT uses microfluidics to ensure gentle single-cell seeding with a clonal recovery rate of up to 80%.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-9bd6d50 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9bd6d50\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-c4fd536\" data-id=\"c4fd536\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f4b0af5 elementor-widget elementor-widget-heading\" data-id=\"f4b0af5\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Research<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e34a79a elementor-widget elementor-widget-text-editor\" data-id=\"e34a79a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMammalian cell lines provide stable models for studying various diseases. Gene editing techniques allow researchers to gain deeper insights into disease mechanisms by facilitating  gene knockout experiments. In vivo studies using mammalian cell lines often serve as pre-clinical justification for human trials<sup>7<\/sup>.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-17718789 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"17718789\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-45740b39\" data-id=\"45740b39\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-332f37b5 elementor-widget elementor-widget-heading\" data-id=\"332f37b5\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Challenges<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6dd30263 elementor-widget elementor-widget-text-editor\" data-id=\"6dd30263\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tDespite the utility of mammalian cell line development, many challenges slow research progress. Overcoming these challenges with advanced easy-to-use instrumentation gives researchers an advantage over the competition. CYTENA provides the automated <a href=\"https:\/\/www.cytena.com\/products\/automated-cell-line-development\/c-station\/\">C.STATION<\/a> platform, an end-to-end solution that solves multiple perennial problems simultaneously.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-9616ca9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9616ca9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-253fafb\" data-id=\"253fafb\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0fb3777 elementor-widget elementor-widget-heading\" data-id=\"0fb3777\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Contamination<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ecf86b8 elementor-widget elementor-widget-text-editor\" data-id=\"ecf86b8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMammalian cell lines are prone to contamination from microbes and other cell lines8. Manual liquid handling increases this risk. Automated liquid handlers like <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">CYTENA\u2019s UP.SIGHT<\/a> reduce contamination risk and ensure monoclonality with &gt;97% single-cell dispensing efficiency.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-cf84088 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"cf84088\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-9ce8226\" data-id=\"9ce8226\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d0e3830 elementor-widget elementor-widget-heading\" data-id=\"d0e3830\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Yield Issues<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e34c095 elementor-widget elementor-widget-text-editor\" data-id=\"e34c095\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tMultiple factors contribute to issues with protein production. Suboptimal cell culture conditions reduce cell productivity and issues with protein folding significantly impact the functionality of the final product. To improve folding, researchers can optimize the protein-coding sequence and co-express chaperone proteins that facilitate proper folding. Researchers can also tailor cell culture conditions, such as temperature, pH, and glucose concentration, to suit the cell line they are using.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-00af32e elementor-widget elementor-widget-text-editor\" data-id=\"00af32e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tSelecting the best clone for scale-up is crucial. <a href=\"https:\/\/www.cytena.com\/products\/reagents-consumables\/f-quant\/\">CYTENA\u2019s F.QUANT<\/a> titer assay facilitates optimal clone selection by allowing researchers to easily measure the production of their target protein.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-44d6c70 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"44d6c70\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-29c2023\" data-id=\"29c2023\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-926f729 elementor-widget elementor-widget-heading\" data-id=\"926f729\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Instability<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e22d74b elementor-widget elementor-widget-text-editor\" data-id=\"e22d74b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell line instability affects therapy manufacturing by diminishing cellular productivity<sup>9<\/sup>. Maintaining stable growing conditions and gentle handling are essential for preventing instability and phenotypic shift. Fully automated cell line development workflows like those achieved through the <a href=\"https:\/\/www.cytena.com\/products\/automated-cell-line-development\/c-station\/\">C.STATION<\/a> help maintain consistency throughout the process and reduce instability.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7076122 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7076122\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-ca59f28\" data-id=\"ca59f28\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cb6e1ae elementor-widget elementor-widget-heading\" data-id=\"cb6e1ae\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Storage<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-43b9988 elementor-widget elementor-widget-text-editor\" data-id=\"43b9988\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tGrowing and scaling up multiple cell lines simultaneously can put strain on laboratory incubators and storage space. Automated single-cell dispensing and microfluidics allow workflows to be scaled down, significantly minimizing storage needs. <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">The UP.SIGHT<\/a> allows researchers to grow clones in 96- and 386-well formats, optimizing both space and resource allocation.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7b499a6 elementor-widget elementor-widget-text-editor\" data-id=\"7b499a6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCheck out our dedicated article on mammalian cell line development for further insights and solutions to common challenges.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7fef8c64 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7fef8c64\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-561704bf\" data-id=\"561704bf\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6727e051 elementor-widget elementor-widget-heading\" data-id=\"6727e051\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Gene Editing in Cell Line Development<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ade68a3 elementor-widget elementor-widget-text-editor\" data-id=\"ade68a3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tAdvances in gene editing have transformed how we approach research, drug discovery and therapy production. New techniques like CRISPR-Cas9 and prime editing are setting new standards for efficiency and accuracy in cell line engineering, paving the way for innovative treatments and research breakthroughs.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-6dd85a1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6dd85a1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-c30b42b\" data-id=\"c30b42b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e333f89 elementor-widget elementor-widget-heading\" data-id=\"e333f89\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Applications<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-651c408 elementor-widget elementor-widget-text-editor\" data-id=\"651c408\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tGene editing can be used to enhance target protein production, stability, and proliferation of cell lines used for therapy production<sup>2<\/sup>. It also enables the creation of universal donor cells and off-the-shelf CAR-T cells for cancer therapy (Fig. 2)<sup>10<\/sup>. Furthermore, gene editing facilitates the reaction of disease models and enables studies of gene function.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d0b5a66 elementor-widget elementor-widget-image\" data-id=\"d0b5a66\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"676\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11-1024x676.jpg\" class=\"attachment-large size-large wp-image-146461\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11-1024x676.jpg 1024w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11-300x198.jpg 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11-768x507.jpg 768w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11-1536x1014.jpg 1536w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11-600x396.jpg 600w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/image-11.jpg 1600w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a996fb6 elementor-widget elementor-widget-text-editor\" data-id=\"a996fb6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<strong>Figure 2.<\/strong> CAR-T cell therapy involves genetically modifying a patient&#8217;s immune cells to recognize and attack cancer cells.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-c497ab8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c497ab8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-0ab32cc\" data-id=\"0ab32cc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-603bf15 elementor-widget elementor-widget-heading\" data-id=\"603bf15\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Methods for Gene Editing<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4b28c64 elementor-widget elementor-widget-text-editor\" data-id=\"4b28c64\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tNovel methods for gene editing are continuously emerging leading to the expansion of applications within cell line development workflows.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7c4d2a8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7c4d2a8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-08a7396\" data-id=\"08a7396\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-add035b elementor-widget elementor-widget-heading\" data-id=\"add035b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">CRISPR-Cas9<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-380fa13 elementor-widget elementor-widget-text-editor\" data-id=\"380fa13\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCRISPR-Cas9 is a groundbreaking gene editing technique that uses guide RNA to direct the Cas9 nuclease to specific genome sequences, causing double-strand breaks for targeted genetic modifications. CRISPR is used to enhance Chinese hamster ovary (CHO) and hybridoma cells for biological therapy production, improving protein yield and cell resilience<sup>2<\/sup>.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-336990f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"336990f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-8bea238\" data-id=\"8bea238\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-20809f3 elementor-widget elementor-widget-heading\" data-id=\"20809f3\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Prime Editing<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-575461f elementor-widget elementor-widget-text-editor\" data-id=\"575461f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tPrime editing uses prime editing guide RNA to direct a fusion protein to specific genomic sites, where it integrates precise DNA sequences via a single-strand nick<sup>11<\/sup>. Though newer and less extensively used, prime editing holds significant promise for optimizing cell line development by enabling precise genetic modifications.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8068276 elementor-widget elementor-widget-text-editor\" data-id=\"8068276\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><a href=\"https:\/\/www.cytena.com\/resource-hub\/blog\/gene-editing-in-cell-line-development-cutting-edge-approaches\/\">Read our dedicated article<\/a> on gene editing for deeper insights and information on other methods of gene editing such as base editing.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-96c7cd1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"96c7cd1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-294c4968\" data-id=\"294c4968\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5a8b9f2d elementor-widget elementor-widget-heading\" data-id=\"5a8b9f2d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Maximizing Efficiency in Cell Line Development<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-63c3a7d1 elementor-widget elementor-widget-text-editor\" data-id=\"63c3a7d1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell line development is crucial for producing modern therapies, and new automation and genetic engineering technologies are enhancing efficiency and precision across development workflows. New cell line development technologies enable companies to bring novel therapeutics to market faster and more efficiently, resulting in significant cost savings and a n edge over the competition.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-fd2fcc3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fd2fcc3\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5d1429d\" data-id=\"5d1429d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2bc30b1 elementor-widget elementor-widget-heading\" data-id=\"2bc30b1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Automation<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0fcd38f elementor-widget elementor-widget-text-editor\" data-id=\"0fcd38f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tAutomation accelerates biomedicine by streamlining cell line development with robotics, AI, and integration of advanced fluid-handling technologies. It enhances efficiency, reduces risks, and frees scientists from repetitive tasks<sup>3<\/sup>. Automation also enables high throughput, allowing efficient seeding, screening, and selection of top-performing clones, thus saving resources and time in scaling up production.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-91a8086 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"91a8086\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-a062993\" data-id=\"a062993\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-514f10c elementor-widget elementor-widget-heading\" data-id=\"514f10c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Quality Control<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b1987a8 elementor-widget elementor-widget-text-editor\" data-id=\"b1987a8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tVerifying single-cell seeding is crucial for regulatory compliance and therapy development success<sup>12<\/sup>. The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT from CYTENA<\/a> images cells during and after dispensing, providing robust evidence of monoclonality with &gt;99.99% clonal derivation and up to 80% clonal recovery. This instrument allows researchers to expedite their workflows while enhancing the quality of their work.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8b5191a elementor-widget elementor-widget-text-editor\" data-id=\"8b5191a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell viability and proliferation are other critical factors in cell line development. The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT<\/a> and <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/c-studio\/\">C.STUDIO<\/a> from CYTENA enable scientists to monitor cell growth, quickly detecting contamination and other issues that affect proliferation and viability.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b6a4d3c elementor-widget elementor-widget-text-editor\" data-id=\"b6a4d3c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tFor more information on optimizing cell line development, including tips on scaling-up and screening, see our dedicated <a href=\"https:\/\/www.cytena.com\/resource-hub\/blog\/maximizing-efficiency-in-cell-line-development-techniques-and-tips\/\">article on maximizing efficiency in cell line development<\/a>.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-065c423 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"065c423\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-e71d3af\" data-id=\"e71d3af\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c104e44 elementor-widget elementor-widget-heading\" data-id=\"c104e44\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Conclusion<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f0bae5a elementor-widget elementor-widget-text-editor\" data-id=\"f0bae5a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tCell line development remains pivotal in advancing biomedical research and therapeutic production. By integrating gene editing and automation innovations, scientists can create more reliable and efficient cell lines in less time. By addressing challenges such as contamination, yield optimization, and cell line stability through cutting-edge technologies like CYTENA\u2019s UP.SIGHT and C.STUDIO, researchers can now achieve higher efficiency and accuracy in cell line development. As these technologies evolve, they promise further to enhance the reliability and effectiveness of cell line development, paving the way for future breakthroughs in biomedicine. CYTENA stands at the forefront of cell line development technologies, helping researchers overcome challenges with advanced, yet simple, solutions.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-aef2808 elementor-widget elementor-widget-text-editor\" data-id=\"aef2808\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tVisit <a href=\"https:\/\/www.cytena.com\/\">our website<\/a> to learn more about our <a href=\"https:\/\/www.cytena.com\/products\/\">comprehensive suite of instruments<\/a> that offer end-to-end solutions for cell line development. Ready to see the power of the UP.SIGHT for yourself? A comprehensive demo is <a href=\"https:\/\/www.cytena.com\/contact-and-support\/\">just one click away<\/a>.\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-98f2311 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"98f2311\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-39e671e\" data-id=\"39e671e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-66f6838 elementor-widget elementor-widget-heading\" data-id=\"66f6838\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">References<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7142fa5 elementor-widget elementor-widget-text-editor\" data-id=\"7142fa5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol>\n<li>O\u2019Flaherty R, Bergin A, Flampouri E, et al. Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing. Biotechnology Advances. 2020;43:107552. doi:10.1016\/j.biotechadv.2020.107552<\/li>\n<li>Shen CC, Sung LY, Lin SY, Lin MW, Hu YC. Enhancing Protein Production Yield from Chinese Hamster Ovary Cells by CRISPR Interference. ACS Synth Biol. 2017;6(8):1509-1519. doi:10.1021\/acssynbio.7b00020<\/li>\n<li>Holland I, Davies JA. Automation in the Life Science Research Laboratory. Front Bioeng Biotechnol. 2020;8:571777. doi:10.3389\/fbioe.2020.571777<\/li>\n<li>Falkenburger BH, Saridaki T, Dinter E. Cellular models for Parkinson\u2019s disease. J Neurochem. 2016;139 Suppl 1:121-130. doi:10.1111\/jnc.13618<\/li>\n<li>Potekhina ES, Bass DY, Kelmanson IV, et al. Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow. Int J Mol Sci. 2020;22(1):148. doi:10.3390\/ijms22010148<\/li>\n<li>Wei F, Wang S, Gou X. A review for cell-based screening methods in drug discovery. Biophys Rep. 2021;7(6):504-516. doi:10.52601\/bpr.2021.210042<\/li>\n<li>Sajjad H, Imtiaz S, Noor T, Siddiqui YH, Sajjad A, Zia M. Cancer models in preclinical research: A chronicle review of advancement in effective cancer research. Animal Model Exp Med. 2021;4(2):87-103. doi:10.1002\/ame2.12165<\/li>\n<li>Weiskirchen S, Schr\u00f6der SK, Buhl EM, Weiskirchen R. A Beginner\u2019s Guide to Cell Culture: Practical Advice for Preventing Needless Problems. Cells. 2023;12(5):682. doi:10.3390\/cells12050682<\/li>\n<li>Wurm F, Wurm M. Cloning of CHO Cells, Productivity and Genetic Stability\u2014A Discussion. Processes. 2017;5(2):20. doi:10.3390\/pr5020020<\/li>\n<li>Poirot L, Philip B, Schiffer-Mannioui C, et al. Multiplex Genome-Edited T-cell Manufacturing Platform for \u201cOff-the-Shelf\u201d Adoptive T-cell Immunotherapies. Cancer Res. 2015;75(18):3853-3864. doi:10.1158\/0008-5472.CAN-14-3321<\/li>\n<li>Zhao Z, Shang P, Mohanraju P, Geijsen N. Prime editing: advances and therapeutic applications. Trends in Biotechnology. 2023;41(8):1000-1012. doi:10.1016\/j.tibtech.2023.03.004<\/li>\n<li>Welch JT, Arden NS. Considering \u201cclonality\u201d: A regulatory perspective on the importance of the clonal derivation of mammalian cell banks in biopharmaceutical development. Biologicals. 2019;62:16-21. doi:10.1016\/j.biologicals.2019.09.006<\/li>\n<\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Linkedin Facebook X-twitter Instagram Cell Line Development: Comprehensive Insights and Advances Cell line development is a cornerstone of several modern biomedical applications. It provides essential models for studying diseases and tools for developing the latest therapeutic products1. Researchers leverage the unique benefits of mammalian cell lines and innovations in gene editing, such as clustered regularly [&hellip;]<\/p>\n","protected":false},"author":34,"featured_media":146443,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[992,991],"tags":[],"products_category":[],"class_list":["post-146442","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-resource-hub"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"Dive into cell line development advancements, from gene editing to automation. 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