{"id":146874,"date":"2024-09-09T09:58:18","date_gmt":"2024-09-09T09:58:18","guid":{"rendered":"https:\/\/www.cytena.com\/?p=146874"},"modified":"2024-10-23T13:16:05","modified_gmt":"2024-10-23T13:16:05","slug":"allogeneic-ips-cell%e2%80%90based-therapy-key-techniques-and-methods","status":"publish","type":"post","link":"https:\/\/www.cytena.com\/resource-hub\/blog\/allogeneic-ips-cell%e2%80%90based-therapy-key-techniques-and-methods\/","title":{"rendered":"Allogeneic iPS Cell\u2010based Therapy: Key Techniques and Methods"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"146874\" class=\"elementor elementor-146874\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-32535686 elementor-section-full_width elementor-section-content-middle elementor-section-height-default elementor-section-height-default\" data-id=\"32535686\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;gradient&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-32cd17b8\" data-id=\"32cd17b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-181c155a elementor-widget__width-initial elementor-widget elementor-widget-post-info\" data-id=\"181c155a\" data-element_type=\"widget\" data-widget_type=\"post-info.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-inline-items elementor-icon-list-items elementor-post-info\">\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-4ac82da elementor-inline-item\" itemprop=\"datePublished\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-date-svg<\/title><style>\t\t.s0 { opacity: .9;fill: #f8fea0 } \t\t.s1 { opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0\" d=\"m0 5.5c0-1.5 1.2-2.8 2.7-2.8h18.6c1.5 0 2.7 1.3 2.7 2.8v2.7h-24z\"><\/path><path id=\"Layer\" class=\"s1\" d=\"m2.7 3.6h18.6c1.1 0 1.9 0.8 1.9 1.9v15.8c0 1.1-0.8 1.9-1.9 1.9h-18.6c-1 0-1.9-0.8-1.9-1.9v-15.8c0-1.1 0.9-1.9 1.9-1.9z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m4.9 0h2.7v2.7h-2.7z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m16.4 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elementor-post-info__item elementor-post-info__item--type-time\">\n\t\t\t\t\t\t\t\t\t\t<time>9:58 am<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38c517fb elementor-widget elementor-widget-heading\" data-id=\"38c517fb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Allogeneic iPS Cell\u2010based Therapy: Key Techniques and Methods<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div data-ep-wrapper-link=\"{&quot;url&quot;:&quot;https:\\\/\\\/www.cytena.com\\\/resource-hub\\\/blog\\\/authors\\\/&quot;,&quot;is_external&quot;:&quot;&quot;,&quot;nofollow&quot;:&quot;&quot;,&quot;custom_attributes&quot;:&quot;&quot;}\" style=\"cursor: pointer\" class=\"bdt-element-link elementor-element elementor-element-714cfb2d elementor-widget elementor-widget-author-box\" data-id=\"714cfb2d\" data-element_type=\"widget\" data-widget_type=\"author-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-author-box\">\n\t\t\t\t\t\t\t<div  class=\"elementor-author-box__avatar\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/Author-1-100x100.png\" alt=\"Picture of By             Adrian Zambrano\" loading=\"lazy\">\n\t\t\t\t<\/div>\n\t\t\t\n\t\t\t<div class=\"elementor-author-box__text\">\n\t\t\t\t\t\t\t\t\t<div >\n\t\t\t\t\t\t<h4 class=\"elementor-author-box__name\">\n\t\t\t\t\t\t\tBy             Adrian Zambrano\t\t\t\t\t\t<\/h4>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-author-box__bio\">\n\t\t\t\t\t\t<p>Customer Success Manager<\/p>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div 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data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-767c0caf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"767c0caf\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5c7763e0\" data-id=\"5c7763e0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-54521cfb elementor-widget elementor-widget-text-editor\" data-id=\"54521cfb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"c4\"><span class=\"c9\">Induced pluripotent stem cells (iPSCs) are an incredibly powerful technology that continues to transform\u00a0<\/span><span class=\"c9\">many facets of biomedicine<\/span><span class=\"c9\">. One particularly exciting area is the use of iPSC-derived cells as therapeutic agents. The\u00a0<\/span><span class=\"c26\">development of allogeneic iPS cell-based therapies<\/span><span class=\"c9\">\u00a0offers \u201coff-the-shelf\u201d therapies for regenerative medicine to treat conditions like cardiovascular disease, retinal degradation, and neurodegenerative disease, to name just a few<\/span><span class=\"c9\">\u00a0(Fig. 1). Despite its therapeutic promise, allogeneic iPS cell\u2010based therapy development is a long and complex process with many challenges and potential pitfalls<\/span><span class=\"c5\">. This article will cover the core steps in developing these therapies, common barriers to success, and future directions in this exciting therapeutic field.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a51ab69 elementor-widget elementor-widget-image\" data-id=\"a51ab69\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"900\" height=\"895\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1.png\" class=\"attachment-full size-full wp-image-146883\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1.png 900w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1-300x298.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1-150x150.png 150w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1-768x764.png 768w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1-600x597.png 600w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog2img1-100x100.png 100w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5d818d1 elementor-widget elementor-widget-text-editor\" data-id=\"5d818d1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figure 1. <\/b><span style=\"font-weight: 400;\">Somatic cells can be reprogrammed to a pluripotent state and then differentiated into different cell types for research and clinical applications.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b72e72e elementor-widget elementor-widget-heading\" data-id=\"b72e72e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Reprogramming and Modification<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-153ca5b elementor-widget elementor-widget-text-editor\" data-id=\"153ca5b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Allogeneic cell therapies differ from autologous therapies in that they are derived from an individual other than the patient and modified to enable their use in virtually any individual<\/span><a href=\"https:\/\/www.zotero.org\/google-docs\/?DpQUUr\"><span style=\"font-weight: 400;\">1<\/span><\/a><span style=\"font-weight: 400;\">. The process begins with the isolation of somatic cells from a consenting donor. These cells are reprogrammed from a differentiated state into a dedifferentiated, pluripotent state through exposure to different transcription factors. These factors, known as the Yamanaka factors, are named after Shinya Yamanaka, who pioneered their use in stem cell research in 2006<\/span><span style=\"font-weight: 400;\">.<\/span><\/p><p><span style=\"font-weight: 400;\">Today, researchers incorporate additional factors beyond those used in the <\/span><span style=\"font-weight: 400;\">early iPSC techniques<\/span><span style=\"font-weight: 400;\"> and several methods are available for delivering these factors to cells, including CRISPR- and RNA-based approaches<\/span><span style=\"font-weight: 400;\">. After reprogramming to become iPSCs, cells can be directed down different lineages to produce diverse cell types. Cells may also be genetically modified depending on the downstream application<\/span><span style=\"font-weight: 400;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f5bd1c8 elementor-widget elementor-widget-heading\" data-id=\"f5bd1c8\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Selection<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-39b07e2 elementor-widget elementor-widget-text-editor\" data-id=\"39b07e2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">The next step is selecting high-quality clones with appropriate iPSC markers, and other desired genetic alterations. Monoclonal populations are required for cell-based therapies, and failure to demonstrate clonality can cause issues with regulatory bodies during drug development. The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\">UP.SIGHT from CYTENA<\/a> provides dual imaging and delicate single-cell dispensing that ensures a &gt;99.99% probability of clonal derivation while preserving the viability of iPSCs, which are sensitive to shear stress (Fig. 2). <br \/><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-019adfb elementor-widget elementor-widget-image\" data-id=\"019adfb\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1000\" height=\"563\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b2img2.png\" class=\"attachment-full size-full wp-image-146884\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b2img2.png 1000w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b2img2-300x169.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b2img2-768x432.png 768w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b2img2-600x338.png 600w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-84f129a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"84f129a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-38b47d1\" data-id=\"38b47d1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-592c941 elementor-widget elementor-widget-text-editor\" data-id=\"592c941\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figure 2: <\/b><span style=\"font-weight: 400;\">The <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT<\/span><\/a><span style=\"font-weight: 400;\"> ensures &gt;97% single-cell dispensing efficiency and up to an 80% clonal recovery rate, helping streamline cell line development workflows.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4de4f8d elementor-widget elementor-widget-heading\" data-id=\"4de4f8d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Quality Control<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-30c8f17 elementor-widget elementor-widget-text-editor\" data-id=\"30c8f17\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Quality control (QC) is an essential part of any therapy development workflow. For iPSC-based therapies, QC is essential for ensuring pluripotency, identifying contaminants, and determining genomic stability. These steps are essential for ensuring the efficacy and safety of the final product and are required by regulatory bodies. QC is performed through a combination of pluripotency tests, genome sequencing, immunogenicity testing, and cell counting to ensure stable growth rates and viability<\/span><span style=\"font-weight: 400;\">. If iPSCs have been induced to differentiate, further functional tests may be performed, such as neuronal tests for synapse formation<\/span><span style=\"font-weight: 400;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-14cd0ce elementor-widget elementor-widget-heading\" data-id=\"14cd0ce\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Scaling Up<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3a41578 elementor-widget elementor-widget-text-editor\" data-id=\"3a41578\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Allogeneic iPSC\u2010based therapies possess the key advantage of being suitable for mass production. Autologous cell therapies are only suitable for a single patient and require smaller, more expensive, and less efficient workflows<\/span><span style=\"font-weight: 400;\">. On the other hand, allogeneic cell therapies can be scaled up and stored for use as \u201coff-the-shelf\u201d treatments for different diseases.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5412ff0 elementor-widget elementor-widget-heading\" data-id=\"5412ff0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Storing<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fab184f elementor-widget elementor-widget-text-editor\" data-id=\"fab184f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Cell banks are an essential part of allogeneic cell-based therapies. They allow therapies to be safely stored and accessed when needed. iPSCs are typically stored using cryopreservation at 196\u00b0C, and protocols usually involve intermediate cooling steps<\/span><span style=\"font-weight: 400;\">. Differentiated cells are also stored in cell banks. Regulatory bodies have strict guidelines for storing these cells, including further rounds of QC before thawed cells are shipped for clinical use and detailed record-keeping to ensure traceability<\/span><span style=\"font-weight: 400;\">.\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ede457a elementor-widget elementor-widget-heading\" data-id=\"ede457a\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Challenges<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f0845f6 elementor-widget elementor-widget-text-editor\" data-id=\"f0845f6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Generating allogeneic iPS cell\u2010based therapies is a complex process with many challenges at each step.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-206f6f3 elementor-widget elementor-widget-text-editor\" data-id=\"206f6f3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><b>Loss of Pluripotency or Other Characteristics<\/b><\/h3><p><span style=\"font-weight: 400;\">iPSCs can lose their pluripotency over time. Loss of pluripotency can occur during the scaling-up phase in large culture volumes and can also occur after thawing<\/span><span style=\"font-weight: 400;\">. Robust QC measures are required to ensure cells are still fit for purpose at different stages of development.<\/span><\/p><p><span style=\"font-weight: 400;\">The <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> can achieve up to 80% clonal recovery for iPSCs without loss of pluripotency. We prepared an application note that showcases the UP.SIGHT\u2019s strengths when handling iPSCs, along with optimization tips for effective iPSC effective culturing.. Read the full document <\/span><a href=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2023\/04\/UP.SIGHT-iPSC-Application-Note.pdf\"><span style=\"font-weight: 400;\">here<\/span><\/a><span style=\"font-weight: 400;\">.<\/span><\/p><h3><b>Immunogenicity of iPSC-derived Allogeneic Cell Therapies<\/b><\/h3><p><span style=\"font-weight: 400;\">Allogeneic cell therapies are derived from donors and face issues with immune rejection when implanted into patients. The HLA (human leukocyte antigen) gene is responsible for allowing cells to recognize self from non-self and is commonly edited to circumvent issues with immunogenicity<\/span><a href=\"https:\/\/www.zotero.org\/google-docs\/?hkQauf\"><span style=\"font-weight: 400;\">2<\/span><\/a><span style=\"font-weight: 400;\">.<\/span><\/p><h3><b>Costs<\/b><\/h3><p><span style=\"font-weight: 400;\">Though more efficient than workflows for developing autologous cell therapies, workflows for developing allogeneic iPSC-based therapies are relatively cost-intensive due to the need for extensive quality control and scalability challenges<\/span><span style=\"font-weight: 400;\">.\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6a7135b elementor-widget elementor-widget-heading\" data-id=\"6a7135b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Future Directions<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-323522e elementor-widget elementor-widget-text-editor\" data-id=\"323522e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">There are many exciting directions in which the <\/span><span style=\"font-weight: 400;\">development of allogeneic iPSC-based therapies<\/span><span style=\"font-weight: 400;\"> is improving, including technologies that expand therapeutic applicability and streamline workflows.<\/span><\/p>\n<h3><b>Combination with Biomaterials and 3D Printing<\/b><\/h3>\n<p><span style=\"font-weight: 400;\">iPSC-derived cell therapies are being combined with 3D biomaterial printing technologies to create novel tissue regeneration products. iPSC-derived organoids are set to play a huge role in drug development and testing, though they have not come to prominence yet<\/span><span style=\"font-weight: 400;\">.<\/span><\/p>\n<h3><b>Automation<\/b><\/h3>\n<p><span style=\"font-weight: 400;\">Automated workflows are already helping to streamline cell line development, including iPSC-derived allogeneic cell therapies. Automation provides faster and more accurate liquid handling and assists in the selection of high-performing clones and the identification of clonal populations<\/span><span style=\"font-weight: 400;\">.&nbsp;<\/span><\/p>\n<p><span style=\"font-weight: 400;\">The <\/span><a href=\"https:\/\/www.cytena.com\/products\/automated-cell-line-development\/c-station\/\"><span style=\"font-weight: 400;\">C.STATION from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> combines the <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT<\/span><\/a><span style=\"font-weight: 400;\"> with our other pioneering cell line development instruments, providing a fully automated end-to-end cell line development workflow.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7693d83 elementor-widget elementor-widget-heading\" data-id=\"7693d83\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Conclusion<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-45df3e4 elementor-widget elementor-widget-text-editor\" data-id=\"45df3e4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Allogeneic iPS cell-based therapies offer immense promise for regenerative medicine, enabling scalable, \u201coff-the-shelf\u201d treatments for various diseases. However, their development is complex, involving reprogramming, selection, rigorous quality control, and overcoming challenges like immune rejection and loss of pluripotency. Despite these hurdles, advancements in automation, quality control, and novel combinations with 3D printing are streamlining the development process and pushing the boundaries of modern healthcare.<\/span><\/p><p><span style=\"font-weight: 400;\">CYTENA is proud to support ambitious companies at the forefront of cell-based therapies. <\/span><a href=\"https:\/\/www.cytena.com\/book-a-demo\/\"><span style=\"font-weight: 400;\">Book a demo<\/span><\/a><span style=\"font-weight: 400;\"> to see firsthand how the <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT<\/span><\/a><span style=\"font-weight: 400;\"> can transform your cell line development workflow.<\/span><\/p><h3><b>References<\/b><\/h3><ol><li><span style=\"font-weight: 400;\">Cerneckis J, Cai H, Shi Y. Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications. <\/span><i><span style=\"font-weight: 400;\">Sig Transduct Target Ther<\/span><\/i><span style=\"font-weight: 400;\">. 2024;9(1):112. doi:10.1038\/s41392-024-01809-0<\/span><\/li><li><span style=\"font-weight: 400;\">Moy AB, Kamath A, Ternes S, Kamath J. The Challenges to Advancing Induced Pluripotent Stem Cell-Dependent Cell Replacement Therapy. <\/span><i><span style=\"font-weight: 400;\">Med Res Arch<\/span><\/i><span style=\"font-weight: 400;\">. 2023;11(11):4784. doi:10.18103\/mra.v11i11.4784<\/span><\/li><li><span style=\"font-weight: 400;\">Takahashi K, Yamanaka S. Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors. <\/span><i><span style=\"font-weight: 400;\">Cell<\/span><\/i><span style=\"font-weight: 400;\">. 2006;126(4):663-676. doi:10.1016\/j.cell.2006.07.024<\/span><\/li><li><span style=\"font-weight: 400;\">Bailly A, Milhavet O, Lemaitre JM. RNA-Based Strategies for Cell Reprogramming toward Pluripotency. <\/span><i><span style=\"font-weight: 400;\">Pharmaceutics<\/span><\/i><span style=\"font-weight: 400;\">. 2022;14(2):317. doi:10.3390\/pharmaceutics14020317<\/span><\/li><li><span style=\"font-weight: 400;\">Xue H, Wu J, Li S, Rao MS, Liu Y. Genetic Modification in Human Pluripotent Stem Cells by Homologous Recombination and CRISPR\/Cas9 System. In: Turksen K, ed. <\/span><i><span style=\"font-weight: 400;\">Human Embryonic Stem Cell Protocols<\/span><\/i><span style=\"font-weight: 400;\">. Vol 1307. Methods in Molecular Biology. Springer New York; 2014:173-190. doi:10.1007\/7651_2014_73<\/span><\/li><li><span style=\"font-weight: 400;\">Madrid M, Lakshmipathy U, Zhang X, et al. Considerations for the development of iPSC-derived cell therapies: a review of key challenges by the JSRM-ISCT iPSC Committee. <\/span><i><span style=\"font-weight: 400;\">Cytotherapy<\/span><\/i><span style=\"font-weight: 400;\">. Published online June 2024:S1465324924007308. doi:10.1016\/j.jcyt.2024.05.022<\/span><\/li><li><span style=\"font-weight: 400;\">Jha BS, Farnoodian M, Bharti K. Regulatory considerations for developing a phase I investigational new drug application for autologous induced pluripotent stem cells-based therapy product. <\/span><i><span style=\"font-weight: 400;\">Stem Cells Transl Med<\/span><\/i><span style=\"font-weight: 400;\">. 2021;10(2):198-208. doi:10.1002\/sctm.20-0242<\/span><\/li><li><span style=\"font-weight: 400;\">Yuan Y, Yang Y, Tian Y, et al. Efficient long-term cryopreservation of pluripotent stem cells at \u221280 \u00b0C. <\/span><i><span style=\"font-weight: 400;\">Sci Rep<\/span><\/i><span style=\"font-weight: 400;\">. 2016;6(1):34476. doi:10.1038\/srep34476<\/span><\/li><li><span style=\"font-weight: 400;\">Chehelgerdi M, Behdarvand Dehkordi F, Chehelgerdi M, et al. Exploring the promising potential of induced pluripotent stem cells in cancer research and therapy. <\/span><i><span style=\"font-weight: 400;\">Mol Cancer<\/span><\/i><span style=\"font-weight: 400;\">. 2023;22(1):189. doi:10.1186\/s12943-023-01873-0<\/span><\/li><li><span style=\"font-weight: 400;\">Soman SS, Vijayavenkataraman S. Applications of 3D Bioprinted-Induced Pluripotent Stem Cells in Healthcare. <\/span><i><span style=\"font-weight: 400;\">Int J Bioprint<\/span><\/i><span style=\"font-weight: 400;\">. 2020;6(4):280. doi:10.18063\/ijb.v6i4.280<\/span><\/li><li><span style=\"font-weight: 400;\">Doulgkeroglou MN, Di Nubila A, Niessing B, et al. Automation, Monitoring, and Standardization of Cell Product Manufacturing. <\/span><i><span style=\"font-weight: 400;\">Front Bioeng Biotechnol<\/span><\/i><span style=\"font-weight: 400;\">. 2020;8:811. doi:10.3389\/fbioe.2020.00811<\/span><\/li><\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Linkedin Facebook X-twitter Instagram Induced pluripotent stem cells (iPSCs) are an incredibly powerful technology that continues to transform\u00a0many facets of biomedicine. One particularly exciting area is the use of iPSC-derived cells as therapeutic agents. The\u00a0development of allogeneic iPS cell-based therapies\u00a0offers \u201coff-the-shelf\u201d therapies for regenerative medicine to treat conditions like cardiovascular disease, retinal degradation, and neurodegenerative [&hellip;]<\/p>\n","protected":false},"author":32,"featured_media":146875,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[992,991],"tags":[],"products_category":[],"class_list":["post-146874","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-resource-hub"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - 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