
{"id":146876,"date":"2024-09-16T10:12:34","date_gmt":"2024-09-16T10:12:34","guid":{"rendered":"https:\/\/www.cytena.com\/?p=146876"},"modified":"2024-10-23T13:20:03","modified_gmt":"2024-10-23T13:20:03","slug":"automation-in-ipsc-based-cell-therapy-generation-increasing-efficiency-and-consistency","status":"publish","type":"post","link":"https:\/\/www.cytena.com\/resource-hub\/blog\/automation-in-ipsc-based-cell-therapy-generation-increasing-efficiency-and-consistency\/","title":{"rendered":"Automation in iPSC-based Cell Therapy Generation: Increasing Efficiency and Consistency"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"146876\" class=\"elementor elementor-146876\" 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data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-32535686 elementor-section-full_width elementor-section-content-middle elementor-section-height-default elementor-section-height-default\" data-id=\"32535686\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;gradient&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-32cd17b8\" data-id=\"32cd17b8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-181c155a elementor-widget__width-initial elementor-widget elementor-widget-post-info\" data-id=\"181c155a\" data-element_type=\"widget\" data-widget_type=\"post-info.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-inline-items elementor-icon-list-items elementor-post-info\">\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-4ac82da elementor-inline-item\" itemprop=\"datePublished\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-date-svg<\/title><style>\t\t.s0 { opacity: .9;fill: #f8fea0 } \t\t.s1 { opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0\" d=\"m0 5.5c0-1.5 1.2-2.8 2.7-2.8h18.6c1.5 0 2.7 1.3 2.7 2.8v2.7h-24z\"><\/path><path id=\"Layer\" class=\"s1\" d=\"m2.7 3.6h18.6c1.1 0 1.9 0.8 1.9 1.9v15.8c0 1.1-0.8 1.9-1.9 1.9h-18.6c-1 0-1.9-0.8-1.9-1.9v-15.8c0-1.1 0.9-1.9 1.9-1.9z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m4.9 0h2.7v2.7h-2.7z\"><\/path><path id=\"Layer\" class=\"s0\" d=\"m16.4 0h2.7v2.7h-2.7z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-date\">\n\t\t\t\t\t\t\t\t\t\t<time>September 16, 2024<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<li class=\"elementor-icon-list-item elementor-repeater-item-dd7bde9 elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewBox=\"0 0 25 25\" width=\"25\" height=\"25\"><title>icon-time-svg<\/title><style>\t\t.s0_{ opacity: .9;fill: none;stroke: #f8fea0;stroke-width: 1.6 } \t<\/style><path id=\"Layer\" class=\"s0_\" d=\"m12.5 23.2c-6.2 0-11.2-5-11.2-11.2 0-6.2 5-11.2 11.2-11.2 6.2 0 11.2 5 11.2 11.2 0 6.2-5 11.2-11.2 11.2z\"><\/path><path id=\"Layer\" fill-rule=\"evenodd\" class=\"s0_\" d=\"m12.2 7.6v4.4l4.4 4.4\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text elementor-post-info__item elementor-post-info__item--type-time\">\n\t\t\t\t\t\t\t\t\t\t<time>10:12 am<\/time>\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38c517fb elementor-widget elementor-widget-heading\" data-id=\"38c517fb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Automation in iPSC-based Cell Therapy Generation: Increasing Efficiency and Consistency<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div data-ep-wrapper-link=\"{&quot;url&quot;:&quot;https:\\\/\\\/www.cytena.com\\\/resource-hub\\\/blog\\\/authors\\\/&quot;,&quot;is_external&quot;:&quot;&quot;,&quot;nofollow&quot;:&quot;&quot;,&quot;custom_attributes&quot;:&quot;&quot;}\" style=\"cursor: pointer\" class=\"bdt-element-link elementor-element elementor-element-714cfb2d elementor-widget elementor-widget-author-box\" data-id=\"714cfb2d\" data-element_type=\"widget\" data-widget_type=\"author-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-author-box\">\n\t\t\t\t\t\t\t<div  class=\"elementor-author-box__avatar\">\n\t\t\t\t\t<img decoding=\"async\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/07\/Author-1-100x100.png\" alt=\"Picture of By             Adrian Zambrano\" loading=\"lazy\">\n\t\t\t\t<\/div>\n\t\t\t\n\t\t\t<div class=\"elementor-author-box__text\">\n\t\t\t\t\t\t\t\t\t<div >\n\t\t\t\t\t\t<h4 class=\"elementor-author-box__name\">\n\t\t\t\t\t\t\tBy             Adrian Zambrano\t\t\t\t\t\t<\/h4>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-author-box__bio\">\n\t\t\t\t\t\t<p>Customer Success Manager<\/p>\n\t\t\t\t\t<\/div>\n\t\t\t\t\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-39d9a17c\" data-id=\"39d9a17c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4638d6d0 elementor-widget elementor-widget-image\" data-id=\"4638d6d0\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"800\" height=\"534\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/10\/1-copiar-6-1.png\" class=\"attachment-large size-large wp-image-146877\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/10\/1-copiar-6-1.png 800w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/10\/1-copiar-6-1-300x200.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/10\/1-copiar-6-1-768x513.png 768w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/10\/1-copiar-6-1-600x401.png 600w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-365c5639 elementor-grid-4 e-grid-align-left social-icon-sticky elementor-widget__width-initial elementor-absolute e-grid-align-mobile-center elementor-shape-rounded elementor-widget elementor-widget-social-icons\" data-id=\"365c5639\" data-element_type=\"widget\" data-settings=\"{&quot;_position&quot;:&quot;absolute&quot;}\" data-widget_type=\"social-icons.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-social-icons-wrapper elementor-grid\">\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a 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class=\"elementor-screen-only\">X-twitter<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-x-twitter\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-instagram elementor-repeater-item-f07f607\" href=\"https:\/\/www.instagram.com\/cytena\/\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Instagram<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-instagram\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-620483af elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"620483af\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-80b7311\" data-id=\"80b7311\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-767c0caf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"767c0caf\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-5c7763e0\" data-id=\"5c7763e0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-54521cfb elementor-widget elementor-widget-text-editor\" data-id=\"54521cfb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Induced pluripotent stem cell (iPSC)-based allogeneic cell therapies are set to transform many fields of healthcare, especially regenerative medicine<\/span><a href=\"https:\/\/www.zotero.org\/google-docs\/?nKtuac\"><span style=\"font-weight: 400;\">1<\/span><\/a><span style=\"font-weight: 400;\">. These therapies mean that donor-derived iPSCs can be modified and scaled up for use as <\/span><span style=\"font-weight: 400;\">\u201coff-the-shelf\u201d<\/span><span style=\"font-weight: 400;\"> treatments for neurodegenerative, cardiovascular, and retinal diseases, to name just a few<\/span><span style=\"font-weight: 400;\">. Automation is also emerging as a core pillar of modern drug development workflows, facilitating efficiency, reliability, scalability, and higher throughput<\/span><span style=\"font-weight: 400;\">. This article will discuss the many benefits of automation and how it leads to more <\/span><span style=\"font-weight: 400;\">efficient iPSC production<\/span><span style=\"font-weight: 400;\"> and accelerates the next generation of allogeneic cell therapies.\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-67a36380 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"67a36380\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-56382804\" data-id=\"56382804\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7f84821e elementor-widget elementor-widget-heading\" data-id=\"7f84821e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Key Technologies in Automation<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b3f27eb elementor-widget elementor-widget-text-editor\" data-id=\"1b3f27eb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automation technologies span the entire cell therapy development workflow, offering enormous benefits at each stage.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-77a1a3f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"77a1a3f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-4f0ee7f4\" data-id=\"4f0ee7f4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-495b568b elementor-widget elementor-widget-heading\" data-id=\"495b568b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\"> Single Cell Dispensing<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1eab2091 elementor-widget elementor-widget-text-editor\" data-id=\"1eab2091\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Obtaining a monoclonal population is a requirement for cell-based therapies. Manual methods for achieving this are time-intensive and often rely on techniques like limiting dilution, which are inherently error-prone. Automation, on the other hand, all but guarantees clonality by integrating single-cell dispensing technology. Microfluidic systems and advanced imaging technologies are central to automation for single-cell dispensing<\/span><span style=\"font-weight: 400;\">. The <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> exemplifies the latest in single-cell dispensing technology and offers researchers &gt;97% single-cell dispensing efficiency.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-5072a011 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5072a011\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-48e82b38\" data-id=\"48e82b38\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6d1846b1 elementor-widget elementor-widget-heading\" data-id=\"6d1846b1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Monitoring<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-46f09c0c elementor-widget elementor-widget-text-editor\" data-id=\"46f09c0c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automation in iPSC generation<\/span><span style=\"font-weight: 400;\"> includes monitoring clonal growth and confluency after seeding. These capabilities allow scientists to collect large amounts of data concerning clonal performance and viability without time-consuming manual tracking. This, in turn, enables better decision-making and saves countless hours of tedious labor (Fig. 1).<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2a40e97 elementor-widget elementor-widget-image\" data-id=\"2a40e97\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"757\" height=\"454\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b3img1.png\" class=\"attachment-full size-full wp-image-146886\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b3img1.png 757w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b3img1-300x180.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/b3img1-600x360.png 600w\" sizes=\"(max-width: 757px) 100vw, 757px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-86f49a3 elementor-widget elementor-widget-text-editor\" data-id=\"86f49a3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figure 1: <\/b><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/c-studio\/\"><span style=\"font-weight: 400;\">CYTENA\u2019s<\/span> <span style=\"font-weight: 400;\">C.STUDIO software<\/span><\/a><span style=\"font-weight: 400;\"> paired with the <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT<\/span><\/a><span style=\"font-weight: 400;\"> facilitates accurate cell counting and user-friendly reporting.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-7fef8c64 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7fef8c64\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-561704bf\" data-id=\"561704bf\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0378753 elementor-widget elementor-widget-heading\" data-id=\"0378753\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Cell Culture<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ade68a3 elementor-widget elementor-widget-text-editor\" data-id=\"ade68a3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automated cell culture allows for the monitoring of cell concentration and the provision of optimal growing conditions through the replenishing of culture media and the coating of well plates. Robotic handling allows iPSCs to be assayed for efficiency and for faster-growing clones to be selected and scaled up<\/span><span style=\"font-weight: 400;\">. Automated cell culture workflows facilitate the integration of seeding technologies and bioreactors for <\/span><span style=\"font-weight: 400;\">efficient iPSC production<\/span><span style=\"font-weight: 400;\"> and expansion, ensuring pluripotent cells with the desired genetic alterations are carried forward. The <\/span><a href=\"https:\/\/www.cytena.com\/products\/automated-cell-line-development\/c-station\/\"><span style=\"font-weight: 400;\">C.STATION from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> integrates high-end instruments to achieve iPSC cell line development within a single automated platform.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-127d470 elementor-widget elementor-widget-heading\" data-id=\"127d470\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Benefits of Automation in iPSC-based Cell Therapy Generation<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3f96fec elementor-widget elementor-widget-text-editor\" data-id=\"3f96fec\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automation enhances virtually every aspect of cell line development workflows, making it an essential consideration for companies looking to pioneer the next breakthrough and get ahead of the competition.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1bf7248 elementor-widget elementor-widget-heading\" data-id=\"1bf7248\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Efficiency<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b0f88e5 elementor-widget elementor-widget-text-editor\" data-id=\"b0f88e5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automation enhances efficiency both in individual tasks and over the longer timelines involved in cell line development<\/span><span style=\"font-weight: 400;\">. Automated liquid handlers perform tasks more accurately and quickly than human operators<\/span><span style=\"font-weight: 400;\">\u00a0and can be programmed to work around the clock to help researchers meet tight deadlines. Researchers can focus on other duties while automated systems perform dispensing tasks, ensuring reproducibility and traceability throughout the cell line development process.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a5a1cea elementor-widget elementor-widget-text-editor\" data-id=\"a5a1cea\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">The <\/span><a href=\"https:\/\/www.cytena.com\/products\/automated-cell-line-development\/c-station\/\"><span style=\"font-weight: 400;\">C.STATION from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> is our complete end-to-end automated cell line development system. It incorporates the <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT<\/span><\/a><span style=\"font-weight: 400;\"> for delicate single-cell dispensing and our other high-end instrumentation to deliver an efficient and robust cell line development workflow.\u00a0<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8fc657f elementor-widget elementor-widget-heading\" data-id=\"8fc657f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Scale<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-36af70e elementor-widget elementor-widget-text-editor\" data-id=\"36af70e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">With automation, researchers can work at a scale that best suits their research and is optimized for resource efficiency<\/span><span style=\"font-weight: 400;\">. Microfluidics systems and precise liquid dispensing mean iPSC-based allogeneic cell therapy workflows can be significantly scaled down to save money on consumables like cell culture medium and multiwell plates. Once the best clones are selected, automation allows these cells to be efficiently scaled up while ensuring optimal growing conditions<\/span><span style=\"font-weight: 400;\">. iPSCs are prone to losing essential characteristics like pluripotency over time<\/span><span style=\"font-weight: 400;\">. Efficient scaling means large batches of high-quality iPSCs can be produced quickly before critical traits are lost.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2d37b14 elementor-widget elementor-widget-heading\" data-id=\"2d37b14\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Reduced Contamination<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5aa9041 elementor-widget elementor-widget-text-editor\" data-id=\"5aa9041\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Contamination in iPSC-based allogeneic cell therapy is incredibly costly. Cell lines are at risk of contamination from microbes like mycoplasma, other cell lines and clones, and cells within their clonal population that have lost pluripotency<\/span><span style=\"font-weight: 400;\">. Each of these scenarios carries unique risks that severely disrupt therapy development pipelines. Automated dispensing and liquid handling significantly reduce the chances of microbial contamination, which is more common with manual methods<\/span><span style=\"font-weight: 400;\">.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-04243b3 elementor-widget elementor-widget-heading\" data-id=\"04243b3\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Reliability<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5e7a102 elementor-widget elementor-widget-text-editor\" data-id=\"5e7a102\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automation in iPSC generation<\/span><span style=\"font-weight: 400;\"> means precise procedures can be repeated within and across laboratories. This reduces the inevitable reproducibility issues that occur with manual methods and multiple human operators. Furthermore, automation provides reliable and accurate dispensing and liquid handling. For example, the <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> uses delicate dispensing to ensure that a single iPSC is dispensed without exposing it to shear forces that can compromise growth rates and viability.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-81bb1b7 elementor-widget elementor-widget-heading\" data-id=\"81bb1b7\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Throughput<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-04ab6f4 elementor-widget elementor-widget-text-editor\" data-id=\"04ab6f4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">High throughput is essential for modern therapy development. Automated dispensing means thousands of single clones can be dispensed and validated quickly without manual input. Monitoring many clones produces high quantities of data that automated computerized systems can collect and manage instantly. The <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> is paired with the dedicated software <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/c-studio\/\"><span style=\"font-weight: 400;\">C.STUDIO<\/span><\/a><span style=\"font-weight: 400;\">, which collects crucial data on clonality and confluency.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-21a5ae8 elementor-widget elementor-widget-heading\" data-id=\"21a5ae8\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">Regulatory Compliance<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8e8074e elementor-widget elementor-widget-text-editor\" data-id=\"8e8074e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">iPSC-based allogeneic cell therapies require robust documentation to achieve regulatory compliance<\/span><span style=\"font-weight: 400;\">. This includes precise information about the source of cells, how they were handled, and proof that the population is monoclonal. Automated systems mean researchers can execute reproducible, standardized workflows for iPSC-based cell therapies<\/span><span style=\"font-weight: 400;\">&nbsp;and provide regulatory bodies with precise protocols that use current good manufacturing practices (cGMP). The <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> has dual imaging technology that images cells as they are being dispensed and after they have settled in the well (Fig. 2). It also monitors clones as they expand, providing robust evidence of clonality that helps to streamline therapy development and avoid the common pitfalls of manual workflows.&nbsp;<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-86388b3 elementor-widget elementor-widget-image\" data-id=\"86388b3\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"690\" height=\"305\" src=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog3img2.png\" class=\"attachment-full size-full wp-image-146887\" alt=\"\" srcset=\"https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog3img2.png 690w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog3img2-300x133.png 300w, https:\/\/www.cytena.com\/wp-content\/uploads\/2024\/09\/blog3img2-600x265.png 600w\" sizes=\"(max-width: 690px) 100vw, 690px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f9b53f5 elementor-widget elementor-widget-text-editor\" data-id=\"f9b53f5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Figure 2: <\/b><span style=\"font-weight: 400;\">The <\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\">UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> facilitates the detection and monitoring of single clones, helping to ensure regulatory compliance.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2a462b2 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2a462b2\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-dc4e61f\" data-id=\"dc4e61f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2880e5b elementor-widget elementor-widget-heading\" data-id=\"2880e5b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Conclusion<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fa79901 elementor-widget elementor-widget-text-editor\" data-id=\"fa79901\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">Automation is revolutionizing iPSC-based allogeneic cell therapy generation by enhancing efficiency, scalability, and reliability. Automated technologies streamline critical processes such as single-cell dispensing, monitoring, and cell culture, ensuring consistent results and reducing contamination risks. By enabling higher throughput and robust documentation, automation supports regulatory compliance and accelerates the development of next-generation therapies. As a result, automation is pivotal in advancing healthcare, paving the way for more breakthroughs in regenerative medicine.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fe9dc18 elementor-widget elementor-widget-text-editor\" data-id=\"fe9dc18\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"font-weight: 400;\">The<\/span><a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/up-sight\/\"><span style=\"font-weight: 400;\"> UP.SIGHT from CYTENA<\/span><\/a><span style=\"font-weight: 400;\"> is a game-changer in cell line development. <\/span><a href=\"https:\/\/www.cytena.com\/book-a-demo\/\"><span style=\"font-weight: 400;\">Schedule a demo today<\/span><\/a><span style=\"font-weight: 400;\"> or <\/span><a href=\"https:\/\/www.cytena.com\/contact-and-support\/\"><span style=\"font-weight: 400;\">contact our team<\/span><\/a><span style=\"font-weight: 400;\"> for more details on this groundbreaking technology.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-925c3f6 elementor-widget elementor-widget-heading\" data-id=\"925c3f6\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">References<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dd406c1 elementor-widget elementor-widget-text-editor\" data-id=\"dd406c1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ol>\n \t<li> <span style=\"font-weight: 400;\">Singh VK, Kalsan M, Kumar N, Saini A, Chandra R. Induced pluripotent stem cells: applications in regenerative medicine, disease modeling, and drug discovery. <\/span><i><span style=\"font-weight: 400;\">Front Cell Dev Biol<\/span><\/i><span style=\"font-weight: 400;\">. 2015;3. doi:10.3389\/fcell.2015.00002<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Cerneckis J, Cai H, Shi Y. Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications. <\/span><i><span style=\"font-weight: 400;\">Sig Transduct Target Ther<\/span><\/i><span style=\"font-weight: 400;\">. 2024;9(1):112. doi:10.1038\/s41392-024-01809-0<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Schneider G. Automating drug discovery. <\/span><i><span style=\"font-weight: 400;\">Nat Rev Drug Discov<\/span><\/i><span style=\"font-weight: 400;\">. 2018;17(2):97-113. doi:10.1038\/nrd.2017.232<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Torres-Acosta MA, Lye GJ, Dikicioglu D. Automated liquid-handling operations for robust, resilient, and efficient bio-based laboratory practices. <\/span><i><span style=\"font-weight: 400;\">Biochem Eng J<\/span><\/i><span style=\"font-weight: 400;\">. 2022;188(108713). doi:10.1016\/j.bej.2022.108713<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Gross A, Schoendube J, Zimmermann S, Steeb M, Zengerle R, Koltay P. Technologies for Single-Cell Isolation. <\/span><i><span style=\"font-weight: 400;\">Int J Mol Sci<\/span><\/i><span style=\"font-weight: 400;\">. 2015;16(8):16897-16919. doi:10.3390\/ijms160816897<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Doulgkeroglou MN, Di Nubila A, Niessing B, et al. Automation, Monitoring, and Standardization of Cell Product Manufacturing. <\/span><i><span style=\"font-weight: 400;\">Front Bioeng Biotechnol<\/span><\/i><span style=\"font-weight: 400;\">. 2020;8:811. doi:10.3389\/fbioe.2020.00811<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Schwedhelm I, Zdzieblo D, Appelt-Menzel A, et al. Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors. <\/span><i><span style=\"font-weight: 400;\">Sci Rep<\/span><\/i><span style=\"font-weight: 400;\">. 2019;9(1):12297. doi:10.1038\/s41598-019-48814-w<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Tristan CA, Ormanoglu P, Slamecka J, et al. Robotic high-throughput biomanufacturing and functional differentiation of\u00a0human pluripotent stem cells. <\/span><i><span style=\"font-weight: 400;\">Stem Cell Reports<\/span><\/i><span style=\"font-weight: 400;\">. 2021;16(12):3076-3092. doi:10.1016\/j.stemcr.2021.11.004<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Holland I, Davies JA. Automation in the Life Science Research Laboratory. <\/span><i><span style=\"font-weight: 400;\">Front Bioeng Biotechnol<\/span><\/i><span style=\"font-weight: 400;\">. 2020;8(571777). doi:10.3389\/fbioe.2020.571777<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">Madrid M, Lakshmipathy U, Zhang X, et al. Considerations for the development of iPSC-derived cell therapies: a review of key challenges by the JSRM-ISCT iPSC Committee. <\/span><i><span style=\"font-weight: 400;\">Cytotherapy<\/span><\/i><span style=\"font-weight: 400;\">. Published online June 2024:S1465324924007308. doi:10.1016\/j.jcyt.2024.05.022<\/span><\/li>\n \t<li> <span style=\"font-weight: 400;\">McKenna DH, Perlingeiro RCR. Development of allogeneic iPS cell-based therapy: from bench to bedside. <\/span><i><span style=\"font-weight: 400;\">EMBO Mol Med<\/span><\/i><span style=\"font-weight: 400;\">. 2023;15(2):e15315. doi:10.15252\/emmm.202115315<\/span><\/li>\n<\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Linkedin Facebook X-twitter Instagram Induced pluripotent stem cell (iPSC)-based allogeneic cell therapies are set to transform many fields of healthcare, especially regenerative medicine1. These therapies mean that donor-derived iPSCs can be modified and scaled up for use as \u201coff-the-shelf\u201d treatments for neurodegenerative, cardiovascular, and retinal diseases, to name just a few. Automation is also emerging [&hellip;]<\/p>\n","protected":false},"author":32,"featured_media":146877,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[992,991],"tags":[],"products_category":[],"class_list":["post-146876","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-resource-hub"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - 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