{"id":148020,"date":"2025-02-11T10:44:31","date_gmt":"2025-02-11T10:44:31","guid":{"rendered":"https:\/\/www.cytena.com\/?post_type=application-notes&#038;p=148020"},"modified":"2025-09-16T11:22:06","modified_gmt":"2025-09-16T11:22:06","slug":"unleashing-single-cell-proteomics-scpico-revolutionizes-absolute-quantification-with-f-sight-i-dot-technology","status":"publish","type":"application-notes","link":"https:\/\/www.cytena.com\/resource-hub\/application-note\/unleashing-single-cell-proteomics-scpico-revolutionizes-absolute-quantification-with-f-sight-i-dot-technology\/","title":{"rendered":"Unleashing single-cell proteomics: scPICO revolutionizes absolute quantification with F.SIGHT\u2122 &amp; I.DOT\u2122 technology"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"148020\" class=\"elementor elementor-148020\" data-elementor-settings=\"{&quot;element_pack_global_tooltip_width&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_width_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;element_pack_global_tooltip_padding&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_padding_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true},&quot;element_pack_global_tooltip_border_radius_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;top&quot;:&quot;&quot;,&quot;right&quot;:&quot;&quot;,&quot;bottom&quot;:&quot;&quot;,&quot;left&quot;:&quot;&quot;,&quot;isLinked&quot;:true}}\" data-elementor-post-type=\"application-notes\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0a574bc e-flex e-con-boxed e-con e-parent\" data-id=\"0a574bc\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-69bc7c2 elementor-widget elementor-widget-text-editor\" data-id=\"69bc7c2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p data-start=\"176\" data-end=\"628\">Single-cell protein quantification is key for exploring cellular heterogeneity but often lacks sensitivity, throughput, or accuracy. The scPICO assay was developed to enable reference-free absolute quantification of proteins at the single-cell level. It combines the <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight\/\">F.SIGHT<\/a>\u00a0for precise single-cell dispensing and the <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT<\/a> for ultra-low-volume reagent handling.<\/p><p data-start=\"176\" data-end=\"628\">The resulting workflow delivers highly sensitive, high-throughput single-cell proteoform analysis with minimal background interference.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-87a0cd3 e-flex e-con-boxed e-con e-parent\" data-id=\"87a0cd3\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d07ab23 elementor-widget elementor-widget-text-editor\" data-id=\"d07ab23\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Learn more about how:<\/strong><\/p><ul><li data-start=\"709\" data-end=\"801\"><p data-start=\"711\" data-end=\"801\">The <a href=\"https:\/\/www.cytena.com\/products\/single-cell-dispensers\/f-sight\/\">F.SIGHT<\/a>\u00a0isolates single cells into 384-well plates with sub-nanoliter precision<\/p><\/li><li data-start=\"802\" data-end=\"895\"><p data-start=\"804\" data-end=\"895\">The <a href=\"https:\/\/www.cytena.com\/products\/liquid-handlers\/i-dot\/\">I.DOT <\/a>performs low-volume dispensing of labeled antibodies in the scPICO workflow<\/p><\/li><li data-start=\"896\" data-end=\"1035\"><p data-start=\"898\" data-end=\"1035\">scPICO enables absolute quantification of proteins and phosphorylation states at single-cell resolution, revealing cellular heterogeneity<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Single-cell protein quantification is key for exploring cellular heterogeneity but often lacks sensitivity, throughput, or accuracy. The scPICO assay was developed to enable reference-free absolute quantification of proteins at the single-cell level. It combines the F.SIGHT\u00a0for precise single-cell dispensing and the I.DOT for ultra-low-volume reagent handling. The resulting workflow delivers highly sensitive, high-throughput single-cell proteoform [&hellip;]<\/p>\n","protected":false},"featured_media":148021,"parent":0,"menu_order":0,"template":"elementor_theme","meta":{"_acf_changed":true,"content-type":"","inline_featured_image":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"tags":[],"application_notes":[976,962],"class_list":["post-148020","application-notes","type-application-notes","status-publish","has-post-thumbnail","hentry","application_notes-rna-sequencing","application_notes-single-cell-omics"],"acf":[],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.3.0 - aioseo.com -->\n\t\t<meta name=\"description\" content=\"Single-cell protein quantification is key for exploring cellular heterogeneity but often lacks sensitivity, throughput, or accuracy. 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